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Review
. 2022 Mar 2:10:794411.
doi: 10.3389/fbioe.2022.794411. eCollection 2022.

An Insight in Developing Carrier-Free Immobilized Enzymes

Vivek Chauhan  1 , Diksha Kaushal  1 , Vivek Kumar Dhiman  1 , Shamsher Singh Kanwar  1 , Devendra Singh  2 , Vinay Kumar Dhiman  3 , Himanshu Pandey  3
Affiliations
Review

An Insight in Developing Carrier-Free Immobilized Enzymes

Vivek Chauhan et al. Front Bioeng Biotechnol. .

Abstract

Enzymes play vital roles in all organisms. The enzymatic process is progressively at its peak, mainly for producing biochemical products with a higher value. The immobilization of enzymes can sometimes tremendously improve the outcome of biocatalytic processes, making the product(s) relatively pure and economical. Carrier-free immobilized enzymes can increase the yield of the product and the stability of the enzyme in biocatalysis. Immobilized enzymes are easier to purify. Due to these varied advantages, researchers are tempted to explore carrier-free methods used for the immobilization of enzymes. In this review article, we have discussed various aspects of enzyme immobilization, approaches followed to design a process used for immobilization of an enzyme and the advantages and disadvantages of various common processes used for enzyme immobilization.

Keywords: biocatalytic process; carrier-free immobilized enzymes; enzymes; immobilization; immobilized enzymes.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Chemical and physical method(s) of immobilization and their characteristics.
FIGURE 2
FIGURE 2
Features of cross-linked enzyme aggregates.
See this image and copyright information in PMC

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