Peptostreptococcus faecalis sp. nov., new bacterial species isolated from healthy indigenous congolese volunteer

Abstract

The Microbial Culturomics Project aiming to discover several bacterial species made it possible to isolate the strain Marseille-P4308^T from a stool sample of a healthy indigenous Congolese volunteer. Strain Marseille-P4308^T is a Gram-positive coccus shaped bacterium that optimally grows at 37 °C. The 16S rRNA gene sequence of the strain has a 96.2% sequence similarity to Peptostreptococcus anaerobius strain NCTC 11460^T (GenBank accession number: NR_042847.1). In addition, the average nucleotide identity of strain Marseille-P4308^T with its closest related species was 71.1%, which was far below the recommended threshold (>95-96%). The genome of the strain Marseille-P4308^T has a length of 2.14 Mbp with G + C content of 30.4 mol%. Based on phenotypic, biochemical, genomic and phylogenetic analysis, strain Marseille-P4308^T (= CSUR P4308 = CECT 9960) clearly appears to be a new species for which the name Peptostreptococcus faecalis sp. nov., is proposed.

Keywords: Culturomics; Gut microbiote; Indigenous congolese; Peptostreptococcus faecalis; Taxonogenomics.

Figure 1

Phylogenetic tree with the 16S rRNA gene sequences indicating the position of Peptostreptococcus faecalis sp. nov. Marseille-P4308^T among other closely related species. Sequences were aligned using MUSCLE, and phylogenetic inferences were obtained using the Neighbor-Joining method [29] within the MEGA X software [15]. Accession numbers are indicated in parenthesis.

Figure 2

Phylogenomic tree basing on genomic sequences of Peptostreptococcus faecalis sp. nov., Marseille-P4308^T and other its related species. Sequences were concatenated with union EMBOSS (https://www.bioinformatics.nl/cgi-bin/emboss/union) and aligned by Mugsy software [30]. The tree was built using iTOL an online bioinformatic tool [31].