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. 2022 Mar 14;8(3):e09102.
doi: 10.1016/j.heliyon.2022.e09102. eCollection 2022 Mar.

Peptostreptococcus faecalis sp. nov., new bacterial species isolated from healthy indigenous congolese volunteer

Fatima Mekhalif  1   2 , Rita Zgheib  2   3 , Jean Akiana  4 , Melhem Bilen  1   3 , Geor Mongo Ndombe  4 , Florence Fenollar  2   5 , Pierre-Edouard Fournier  2   5 , Didier Raoult  1   2 , Stéphane Alibar  2 , Oleg Mediannikov  1   2 , Cheikh Ibrahima Lo  2   5
Affiliations

Peptostreptococcus faecalis sp. nov., new bacterial species isolated from healthy indigenous congolese volunteer

Fatima Mekhalif et al. Heliyon. .

Abstract

The Microbial Culturomics Project aiming to discover several bacterial species made it possible to isolate the strain Marseille-P4308T from a stool sample of a healthy indigenous Congolese volunteer. Strain Marseille-P4308T is a Gram-positive coccus shaped bacterium that optimally grows at 37 °C. The 16S rRNA gene sequence of the strain has a 96.2% sequence similarity to Peptostreptococcus anaerobius strain NCTC 11460T (GenBank accession number: NR_042847.1). In addition, the average nucleotide identity of strain Marseille-P4308T with its closest related species was 71.1%, which was far below the recommended threshold (>95-96%). The genome of the strain Marseille-P4308T has a length of 2.14 Mbp with G + C content of 30.4 mol%. Based on phenotypic, biochemical, genomic and phylogenetic analysis, strain Marseille-P4308T (= CSUR P4308 = CECT 9960) clearly appears to be a new species for which the name Peptostreptococcus faecalis sp. nov., is proposed.

Keywords: Culturomics; Gut microbiote; Indigenous congolese; Peptostreptococcus faecalis; Taxonogenomics.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Phylogenetic tree with the 16S rRNA gene sequences indicating the position of Peptostreptococcus faecalis sp. nov. Marseille-P4308T among other closely related species. Sequences were aligned using MUSCLE, and phylogenetic inferences were obtained using the Neighbor-Joining method [29] within the MEGA X software [15]. Accession numbers are indicated in parenthesis.
Figure 2
Figure 2
Phylogenomic tree basing on genomic sequences of Peptostreptococcus faecalis sp. nov., Marseille-P4308T and other its related species. Sequences were concatenated with union EMBOSS (https://www.bioinformatics.nl/cgi-bin/emboss/union) and aligned by Mugsy software [30]. The tree was built using iTOL an online bioinformatic tool [31].
See this image and copyright information in PMC

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