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. 2022 Aug;68(3-4):505-514.
doi: 10.1007/s00294-022-01235-1. Epub 2022 Mar 22.

Molecular basis of cycloheximide resistance in the Ophiostomatales revealed

Brenda D Wingfield  1 , Mike J Wingfield  2 , Tuan A Duong  2
Affiliations

Molecular basis of cycloheximide resistance in the Ophiostomatales revealed

Brenda D Wingfield et al. Curr Genet. 2022 Aug.

Abstract

Resistance to the antibiotic Cycloheximide has been reported for a number of fungal taxa. In particular, some yeasts are known to be highly resistant to this antibiotic. Early research showed that this resulted from a transition mutation in one of the 60S ribosomal protein genes. In addition to the yeasts, most genera and species in the Ophiostomatales are highly resistant to this antibiotic, which is widely used to selectively isolate these fungi. Whole-genome sequences are now available for numerous members of the Ophiostomatales providing an opportunity to determine whether the mechanism of resistance in these fungi is the same as that reported for yeast genera such as Kluyveromyces. We examined all the available genomes for the Ophiostomatales and discovered that a transition mutation in the gene coding for ribosomal protein eL42, which results in the substitution of the amino acid Proline to Glutamine, likely confers resistance to this antibiotic. This change across all genera in the Ophiostomatales suggests that the mutation arose early in the evolution of these fungi.

Keywords: Cycloheximide resistance; Ophiostoma; Ophiostomatoid; Ribosomal protein el42.

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References

    1. Aylward J, Steenkamp ET, Dreyer LL, Roets F, Wingfield BD et al (2016) Genome sequences of Knoxdaviesia capensis and K. proteae (Fungi: Ascomycota) from Protea trees in South Africa. Stand Genomic Sci 11:22. https://doi.org/10.1186/s40793-016-0139-9 - DOI - PubMed - PMC
    1. Ban N, Beckmann R, Cate JH, Dinman JD, Dragon F (2014) A new system for naming ribosomal proteins. Curr Opin Struct Biol. https://doi.org/10.1016/j.sbi.2014.01.002 - DOI - PubMed - PMC
    1. Berka RM, Grigoriev IV, Otillar R, Salamov A, Grimwood J et al (2011) Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris. Nat Biotechnol. https://doi.org/10.1038/nbt.1976 - DOI - PubMed
    1. Blanco-Ulate B, Rolshausen P, Cantu D (2013) Draft Genome Sequence of the Ascomycete Phaeoacremonium aleophilum Strain UCR-PA7, a causal agent of the Esca disease complex in grapevines. Genome Announc. https://doi.org/10.1128/genomeA.00390-13 - DOI - PubMed - PMC
    1. Brasch J, Beck-Jendroschek V, Iturrieta-González I, Voss K, Gené J (2019) A human subcutaneous infection by Microascus ennothomasiorum sp. nov. Mycoses 62:157–164. https://doi.org/10.1111/myc.12861 - DOI - PubMed

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