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. 2022 Apr 19;66(4):e0194721.
doi: 10.1128/aac.01947-21. Epub 2022 Mar 22.

Tn 560, a Novel Tn 554 Family Transposon from Porcine Methicillin-Resistant Staphylococcus aureus ST398, Carries a Multiresistance Gene Cluster Comprising a Novel spc Gene Variant and the Genes lsa(E) and lnu(B)

Xing Ji #  1   2 , Henrike Krüger #  3   4 , Yaxin Wang  1 , Andrea T Feßler  3   4 , Yang Wang  1 , Stefan Schwarz  1   3   4 , Congming Wu  1
Affiliations

Tn 560, a Novel Tn 554 Family Transposon from Porcine Methicillin-Resistant Staphylococcus aureus ST398, Carries a Multiresistance Gene Cluster Comprising a Novel spc Gene Variant and the Genes lsa(E) and lnu(B)

Xing Ji et al. Antimicrob Agents Chemother. .
No abstract available

Keywords: PLSA resistance; Staphylococcus aureus; lsa(E); transposon.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIG 1
FIG 1
(a) Comparison of Tn560, Tn554, and a part of plasmid pE508-2. The areas of >99% identity are shaded in gray. Antimicrobial resistance genes are shown in red, transposase genes are in blue, and hypothetical proteins are in gray. The positions of the two recombination sites A and B are indicated by circles. (b) Detailed structures of the two recombination sites A and B. Vertical bars indicate identical bases with respect to the Tn560 sequence. The positions refer to the corresponding database entries (GenBank accession numbers are in parentheses). The boxed areas indicate the areas where the crossover most likely took place.
FIG 2
FIG 2
Detection of the 460-bp amplicon obtained from the circular intermediate of Tn560 that has been formed after the excision of Tn560 from the chromosomal DNA. Lanes M contain the GeneRuler 100-bp Plus DNA ladder (Thermo Fisher Scientific, Dreieich, Germany), lane + contains the PCR amplicon obtained from S. aureus GDC6P096P, and lane − is the negative control, i.e., demineralized water. The sizes of selected fragments of the DNA ladder are indicated in base pairs.
See this image and copyright information in PMC

References

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