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. 2022 Jun;49(6):4909-4917.
doi: 10.1007/s11033-022-07351-y. Epub 2022 Mar 22.

Impact of superovulation and in vitro fertilization on LINE-1 copy number and telomere length in C57BL/6 J mice blastocysts

Thalita S Berteli  1   2   3 , Fang Wang  4 , Fabiana B Kohlrausch  4   5 , Caroline M Da Luz  6   7 , Fernanda V Oliveira  6 , David L Keefe  4 , Paula A Navarro  6   7
Affiliations

Impact of superovulation and in vitro fertilization on LINE-1 copy number and telomere length in C57BL/6 J mice blastocysts

Thalita S Berteli et al. Mol Biol Rep. 2022 Jun.

Abstract

Objective: Millions of babies have been conceived by IVF, yet debate about its safety to offspring continues. We hypothesized that superovulation and in vitro fertilization (IVF) promote genomic changes, including altered telomere length (TL) and activation of the retrotransposon LINE-1 (L1), and tested this hypothesis in a mouse model.

Material and methods: Experimental study analyzing TL and L1 copy number in C57BL/6 J mouse blastocysts in vivo produced from natural mating cycles (N), in vivo produced following superovulation (S), or in vitro produced following superovulation (IVF). We also examined the effects of prolonged culture on TL and L1 copy number in the IVF group comparing blastocysts cultured 96 h versus blastocysts cultured 120 h. TL and L1 copy number were measured by Real Time PCR.

Results: TL in S (n = 77; Mean: 1.50 ± 1.15; p = 0.0007) and IVF (n = 82; Mean: 1.72 ± 1.44; p < 0.0001) exceeded that in N (n = 16; Mean: 0.61 ± 0.27). TL of blastocysts cultured 120 h (n = 15, Mean: 2.14 ± 1.05) was significantly longer than that of embryos cultured for 96 h (n = 67, Mean: 1.63 ± 1.50; p = 0.0414). L1 copy number of blastocysts cultured for 120 h (n = 15, Mean: 1.71 ± 1.49) exceeded that of embryos cultured for 96 h (n = 67, Mean: 0.95 ± 1.03; p = 0.0162).

Conclusions: Intriguingly ovarian stimulation, alone or followed by IVF, produced embryos with significantly longer telomeres compared to in vivo, natural cycle-produced embryos. The significance of this enriched telomere endowment for the health and longevity of offspring born from IVF merit future studies.

Keywords: IVF; L1 copy number; Mice blastocysts; Superovulation; Telomeres.

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References

    1. Iqbal K, Kues WA, Baulain U, Garrels W, Herrmann D, Niemann H (2011) Species-specific telomere length differences between blastocyst cell compartments and ectopic telomere extension in early bovine embryos by human telomerase reverse transcriptase. Biol Reprod 84:723–733. https://doi.org/10.1095/biolreprod.110.087205 - DOI - PubMed
    1. O’Sullivan RJ, Karlseder J (2010) Telomeres: protecting chromosomes against genome instability. Nat Rev Mol Cell Biol 11:171–181. https://doi.org/10.1038/nrm2848 - DOI - PubMed - PMC
    1. Ozturk S, Sozen B, Demir N (2014) Telomere length and telomerase activity during oocyte maturation and early embryo development in mammalian species telomeres includes. Mol Hum Reprod 20:15–30. https://doi.org/10.1093/molehr/gat055 - DOI - PubMed
    1. Vasilopoulos E, Fragkiadaki P, Kalliora C, Fragou D, Docea AO, Vakonaki E, Tsoukalas D, Calina D, Buga AM, Georgiadis G, Mamoulakis C, Makrigiannakis A, Spandidos DA, Tsatsakis A (2019) The association of female and male infertility with telomere length (Review). Int J Mol Med 44:375–389. https://doi.org/10.3892/ijmm.2019.4225 - DOI - PubMed - PMC
    1. Armstrong CA, Tomita K. Fundamental mechanisms of telomerase action in yeasts and mammals: understanding telomeres and telomerase in cancer cells. Open Biol https://doi.org/10.1098/rsob.160338 .

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