Production of Inflammatory Mediators in Conditioned Medium of Adipose Tissue-Derived Mesenchymal Stem Cells (ATMSC)-Treated Fresh Frozen Plasma

Abstract

BACKGROUND Inflammation is the body's first response to an illness that causes irritation or infection. Inflammation is tightly correlated with aging, which is a progressive degenerative process. Conditioned medium (CM) from adipose tissue-derived mesenchymal stem cells (CM-ATMSCs) has been shown to stimulate collagen synthesis and dermal fibroblast migration, as well as reduce wrinkles and improve wound healing. This study aimed to observe the production of inflammatory modulators - interleukin (IL)-1alpha, IL-6, IL-10, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) - in CM-ATMSCs treated with fresh frozen plasma (FFP) at passages 3 (P3), 7, 11, and 15. MATERIAL AND METHODS ATMSCs P3 were obtained from liposuction of female donors, and the CM from ATMSCs was collected. Measurement of these cytokines was performed with ELISA. RESULTS At many passages, IL-6, a proinflammatory modulator, was discovered to be the most powerful modulator among FFP- and non-FFP-treated cells. However, CM-ATMSCs treated with FFP and in the late passage have significant differences (P<0.05) compared to non-FFP treatments and in other passages in their effects on secretion of inflammatory modulators. CONCLUSIONS In conclusion, CM-ATMSC has the potential to secrete proinflammatory modulators.

Figure 1

The percentage of surface marker ATMSCs. CD90=99.57%, CD105=99.52%, CD73=99.74%, CD44=99.32%.

Figure 2

The concentration of inflammatory modulators (IL-1α, IL-6, IL-10, and NF-κB) treated with FFP and non-FFP at passages 3 (A), 7 (B), 11 (C), and 15 (D). * Significant difference in modulators at different treatments (P<0.05) as analyzed by independent-samples t test.

Figure 3

The potential mechanisms of CM-ATMSC with FFP supplementation treatment in an inflammation process. FFP – fresh frozen plasma; NF-κB – nuclear factor kappa-light-chain-enhancer of activated B cell; IL – interleukin.