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. 2022 May;36(5):e24345.
doi: 10.1002/jcla.24345. Epub 2022 Mar 22.

Oxidative stress in patients with asthma and its relation to uncontrolled asthma

Mariam Ammar  1 , Najla Bahloul  2 , Oumayma Amri  1 , Ribh Omri  1 , Hanene Ghozzi  1 , Samy Kammoun  2 , Khaled Zeghal  1 , Lobna Ben Mahmoud  1
Affiliations

Oxidative stress in patients with asthma and its relation to uncontrolled asthma

Mariam Ammar et al. J Clin Lab Anal. 2022 May.

Abstract

This study aims to evaluate markers of oxidative stress in Tunisian asthmatic patients and investigate whether their markers are correlated with uncontrolled asthma. This prospective cohort study was conducted on 48 healthy subjects and 60 patients with asthma (34 patients with controlled asthma and 26 patients with uncontrolled asthma). The levels of malondialdehyde (MDA), advanced oxidation protein products (AOPP), and glutathione (GSH), as well as the activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD), were estimated in plasma by spectrophotometry. Asthmatic patients have significantly higher plasmatic levels of MDA and AOPP than healthy controls (p < 0.001). Lower GSH level and GPx activity were found in patients with asthma compared to controls (p < 0.001). In contrast, higher SOD activity was noted in asthmatic patients (p < 0.001). The comparison among the patients with controlled asthma and uncontrolled asthma revealed increased MDA and AOPP levels and SOD activity (p < 0.001) as well as a decreased GSH level and GPx activity (p = 0.004, p = 0.019) in patients with uncontrolled asthma. Spirometry level was significantly correlated with SOD activity (r = 0.447; p = 0.010), whereas no significant correlations were found with the other parameters (MDA, AOPP, GSH, and GPx). Asthmatic patients, especially those with uncontrolled asthma, suffer a high degree of reactive oxygen species (ROS) formation causing considerable oxidative stress. Increased MDA level and SOD activity and reduced GPx activity were predictors of poorly controlled asthma.

Keywords: asthma; malondialdehyde; oxidative stress; superoxide dismutase; uncontrolled asthma.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

FIGURE 1
FIGURE 1
Oxidant plasma levels in asthmatic patients versus healthy controls and in controlled asthma versus uncontrolled asthma. Each column represents mean ± SD
FIGURE 2
FIGURE 2
Antioxidant enzymatic and non‐enzymatic defenses in asthmatic patients versus healthy controls and in controlled asthma versus uncontrolled asthma. Each column represents mean ± SD
FIGURE 3
FIGURE 3
(A) Correlation between MDA level and spirometry in controlled asthma and uncontrolled asthma patients. (B) Correlation between AOPP level and spirometry in controlled asthma and uncontrolled asthma patients. (C) Correlation between GSH level and spirometry in controlled asthma and uncontrolled asthma patients. (D) Correlation between GPx activity and spirometry in controlled asthma and uncontrolled asthma patients. (E) Correlation between SOD activity and spirometry in controlled asthma and uncontrolled asthma patients
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References

    1. Barnes PJ. New concepts in the pathogenesis of bronchial hyperresponsiveness and asthma. J Allergy Clin Immunol. 1989;83:1013‐1026. - PubMed
    1. Shifren A, Witt C, Christie C, Castro M. Mechanisms of remodeling in asthmatic airways. J Allergy (Cairo). 2012;2012:316049. - PMC - PubMed
    1. Global Strategy for Asthma Management and Prevention . Global Initiative for Asthma (GINA). https://ginasthma.org/wp‐content/uploads/2020/06/GINA‐2020‐report_20_06_...
    1. Nafti S, Taright S, El Ftouh M, et al. Prevalence of asthma in North Africa: the Asthma Insights and Reality in the Maghreb (AIRMAG) study. Respir Med. 2009;103:S2‐S11. - PubMed
    1. Pereira E, Cavalcante A, Lucas P, Holanda M. Asthma control and quality of life in patients with moderate or severe asthma. J Bras Pneumol. 2011;37(6):705‐711. - PubMed