Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle

doi:10.1007/978-1-0716-2055-7_36

. 2022:2442:663-683.

doi: 10.1007/978-1-0716-2055-7_36.

Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle

Affiliations

¹ Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
² Joint Program in Transfusion Medicine, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
³ Harvard Glycomics Center, Harvard Medical School, Boston, MA, USA.
⁴ Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA. pvanry@chem.byu.edu.
⁵ Department of Biochemistry, Brigham Young University, Provo, UT, USA. pvanry@chem.byu.edu.

PMID: 35320552
DOI: 10.1007/978-1-0716-2055-7_36

Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle

Mary L Vallecillo-Zúniga et al. Methods Mol Biol. 2022.

. 2022:2442:663-683.

doi: 10.1007/978-1-0716-2055-7_36.

Authors

Affiliations

¹ Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA.
² Joint Program in Transfusion Medicine, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
³ Harvard Glycomics Center, Harvard Medical School, Boston, MA, USA.
⁴ Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA. pvanry@chem.byu.edu.
⁵ Department of Biochemistry, Brigham Young University, Provo, UT, USA. pvanry@chem.byu.edu.

PMID: 35320552
DOI: 10.1007/978-1-0716-2055-7_36

Abstract

Galectin-1 is a small (14.5 kDa) multifunctional protein with cell-cell and cell-ECM adhesion due to interactions with the carbohydrate recognition domain (CRD). In two types of muscular dystrophies, this lectin protein has shown therapeutic properties, including positive regulation of skeletal muscle differentiation and regeneration. Both Duchenne and limb-girdle muscular dystrophy 2B (LGMD2B) are subtypes of muscular dystrophies characterized by deficient membrane repair, muscle weakness, and eventual loss of ambulation. This chapter explains confocal techniques such as laser injury, calcium imaging, and galectin-1 localization to examine the effects of galectin-1 on membrane repair in injured LGMD2B models.

Keywords: Galectin-1; LGMD2B; Localization; Membrane repair; Muscular dystrophy; Protein labeling; Sarcolemma.

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References

1. Camby I, Le Mercier M, Lefranc F, Kiss R (2006) Galectin-1: a small protein with major functions. Glycobiology 16(11):137R–157R. https://doi.org/10.1093/glycob/cwl025 - DOI - PubMed
1. Vallecillo-Zúniga ML, Rathgeber MF, Poulson PD, Hayes S, Luddington JS, Gill HN, Teynor M, Kartchner BC, Valdoz J, Stowell C (2020) Treatment with galectin-1 improves myogenic potential and membrane repair in dysferlin-deficient models. PLoS One 15(9):e0238441 - DOI
1. Van Ry PM, Wuebbles RD, Key M, Burkin DJJMT (2015) Galectin-1 protein therapy prevents pathology and improves muscle function in the mdx mouse model of Duchenne muscular dystrophy. Mol Ther 23(8):1285–1297 - DOI
1. Wuebbles RD, Cruz V, Van Ry P, Barraza-Flores P, Brewer PD, Jones P, Burkin DJ (2019) Human Galectin-1 Improves Sarcolemma Stability and Muscle Vascularization in the mdx Mouse Model of Duchenne Muscular Dystrophy. Mol Ther Methods Clin Dev 13:145–153. https://doi.org/10.1016/j.omtm.2019.01.004 - DOI - PubMed - PMC
1. Chandra G, Defour A, Mamchoui K, Pandey K, Mishra S, Mouly V, Sreetama S, Ahmad MM, Mahjneh I, Morizono H (2019) Dysregulated calcium homeostasis prevents plasma membrane repair in Anoctamin 5/TMEM16E-deficient patient muscle cells. Cell Death Dis 5(1):1–15

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[1] Camby I, Le Mercier M, Lefranc F, Kiss R (2006) Galectin-1: a small protein with major functions. Glycobiology 16(11):137R–157R. https://doi.org/10.1093/glycob/cwl025 - DOI - PubMed

[2] Camby I, Le Mercier M, Lefranc F, Kiss R (2006) Galectin-1: a small protein with major functions. Glycobiology 16(11):137R–157R. https://doi.org/10.1093/glycob/cwl025 - DOI - PubMed

[3] Vallecillo-Zúniga ML, Rathgeber MF, Poulson PD, Hayes S, Luddington JS, Gill HN, Teynor M, Kartchner BC, Valdoz J, Stowell C (2020) Treatment with galectin-1 improves myogenic potential and membrane repair in dysferlin-deficient models. PLoS One 15(9):e0238441 - DOI

[4] Vallecillo-Zúniga ML, Rathgeber MF, Poulson PD, Hayes S, Luddington JS, Gill HN, Teynor M, Kartchner BC, Valdoz J, Stowell C (2020) Treatment with galectin-1 improves myogenic potential and membrane repair in dysferlin-deficient models. PLoS One 15(9):e0238441 - DOI

[5] Van Ry PM, Wuebbles RD, Key M, Burkin DJJMT (2015) Galectin-1 protein therapy prevents pathology and improves muscle function in the mdx mouse model of Duchenne muscular dystrophy. Mol Ther 23(8):1285–1297 - DOI

[6] Van Ry PM, Wuebbles RD, Key M, Burkin DJJMT (2015) Galectin-1 protein therapy prevents pathology and improves muscle function in the mdx mouse model of Duchenne muscular dystrophy. Mol Ther 23(8):1285–1297 - DOI

[7] Wuebbles RD, Cruz V, Van Ry P, Barraza-Flores P, Brewer PD, Jones P, Burkin DJ (2019) Human Galectin-1 Improves Sarcolemma Stability and Muscle Vascularization in the mdx Mouse Model of Duchenne Muscular Dystrophy. Mol Ther Methods Clin Dev 13:145–153. https://doi.org/10.1016/j.omtm.2019.01.004 - DOI - PubMed - PMC

[8] Wuebbles RD, Cruz V, Van Ry P, Barraza-Flores P, Brewer PD, Jones P, Burkin DJ (2019) Human Galectin-1 Improves Sarcolemma Stability and Muscle Vascularization in the mdx Mouse Model of Duchenne Muscular Dystrophy. Mol Ther Methods Clin Dev 13:145–153. https://doi.org/10.1016/j.omtm.2019.01.004 - DOI - PubMed - PMC

[9] Chandra G, Defour A, Mamchoui K, Pandey K, Mishra S, Mouly V, Sreetama S, Ahmad MM, Mahjneh I, Morizono H (2019) Dysregulated calcium homeostasis prevents plasma membrane repair in Anoctamin 5/TMEM16E-deficient patient muscle cells. Cell Death Dis 5(1):1–15

[10] Chandra G, Defour A, Mamchoui K, Pandey K, Mishra S, Mouly V, Sreetama S, Ahmad MM, Mahjneh I, Morizono H (2019) Dysregulated calcium homeostasis prevents plasma membrane repair in Anoctamin 5/TMEM16E-deficient patient muscle cells. Cell Death Dis 5(1):1–15

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Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle

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Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle

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