[Effect of various factors on the biosynthesis of alkaloids in a culture of Claviceps CP II]

Abstract

The effect of a carbohydrate medium component, aeration, tryptophane and Tween additions on the biosynthesis of alkaloids by Claviceps CP II was being studied. The quantity of synthesized alkaloids and the composition of produced alkaloids depended on the nature of the carbohydrate and its concentration. A few alkaloids were accumulated on media containing xylose, lactose and glucose, whereas active production of alkaloids was observed when galactose maltose, sucrose and sorbit were used. Intensified aeration and introduction of Tween-80 and Tween-40 resulted in an increased alkaloid yield. Exogenous tryptophane had slight stimulatory effect on alkaloid production.