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. 2022 Mar 10;65(1):105-111.
doi: 10.5194/aab-65-105-2022. eCollection 2022.

TIMP1 may affect goat prolificacy by regulating biological function of granulosa cells

Lei Hong  1   2   3 , Xiang Chen  1   2   3 , Min Zhu  1   2   3 , Zheng Ao  1   2   3 , Wen Tang  1   2   3 , Zhinan Zhou  1   2   3
Affiliations

TIMP1 may affect goat prolificacy by regulating biological function of granulosa cells

Lei Hong et al. Arch Anim Breed. .

Abstract

Tissue inhibitor of metalloproteinase 1 (TIMP1) is associated with animal reproductive processes, such as follicular growth, ovulation, luteinization, and embryo development in mammals. The purposes of this study were to explore the expression and localization of TIMP1 in the ovarian tissues and determine the effect of TIMP1 on the function of granulosa cells and the association of TIMP1 with lambing-related genes of the goats. Immunohistochemical analysis showed that TIMP1 protein was strongly expressed by granulosa cells. Enzyme-linked immunosorbent assay (ELISA) results showed that TIMP1 overexpression promoted the secretion of estradiol of granulosa cells after 12, 24, and 48 h of transfection. Moreover, in vitro experiments indicated that TIMP1 had the ability to promote the cell proliferation and elevate the transcriptional levels of four genes associated with goat prolificacy, including BMPR-1B, BMP15, GDF9, and FSHB, in granulosa cells. In conclusion, TIMP1 could be an important molecule in regulating reproductive performance of the goats by affecting estrogen secretion and cell proliferation, as well as the expression of lambing-related genes of granulosa cells in the goats.

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Conflict of interest statement

The contact author has declared that neither they nor their co-authors have any competing interests.

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