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. 2022 Mar 4;9(3):116.
doi: 10.3390/vetsci9030116.

Humoral Responses and Ex Vivo IFN-γ Production after Canine Whole Blood Stimulation with Leishmania infantum Antigen or KMP11 Recombinant Protein

Pamela Martínez-Orellana  1 , Noemí González  1 , Antonella Baldassarre  2 , Alejandra Álvarez-Fernández  1 , Laura Ordeix  1 , Paola Paradies  2 , Manuel Soto  3 , Laia Solano-Gallego  1
Affiliations

Humoral Responses and Ex Vivo IFN-γ Production after Canine Whole Blood Stimulation with Leishmania infantum Antigen or KMP11 Recombinant Protein

Pamela Martínez-Orellana et al. Vet Sci. .

Abstract

The effect of Leishmania infantum soluble antigen (LSA) and recombinant Kinetoplastid Membrane Protein 11 (rKMP11) on the induction of ex vivo specific IFN-γ (n = 69) and antibody responses (n = 108) was determined in dogs. All dogs were tested for serological response to both antigens and divided into Group 1: healthy (Asturias, Spain, n = 26), Group 2: sick (n = 46), Group 3: healthy Ibizan hounds (Mallorca, Spain, n = 22) and Group 4: healthy (Bari, Italy, n = 14). Antibody levels were higher for LSA when compared to rKMP11 (p = 0.001). Ibizan hounds were all seronegative to rKMP11 and 18% were low seropositive to LSA. Sick dogs presented higher antibody response to both antigens compared to the rest of the groups (p < 0.0001). All groups showed higher IFN-γ levels after LSA compared to rKMP11 responses (p < 0.05). The highest response to LSA was found in Ibizan hounds (p < 0.05). IFN-γ to LSA and rKMP11 stimulation was observed in 34% and in 2.8% of the sick dogs, respectively. Here, we demonstrated that anti-rKMP11 antibodies are mainly present in dogs with moderate to severe disease. Furthermore, cellular immune response measured by specific ex vivo IFN-γ production was more intense to LSA than stimulated to rKMP11.

Keywords: cell-mediated immunity; diagnosis; dog; leishmaniosis; serology.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Specific (a) L infantum and (b) rKMP11 antibodies levels in apparently healthy non-infected dogs from a low endemicity area, Asturias, Spain (Group 1), sick dogs from Catalonia, Spain (Group 2) apparently healthy Ibizan hounds from a high endemicity area, Mallorca, Spain (Group 3) and apparently healthy dogs from a high endemicity area, Bari, Italy (Group 4).
Figure 2
Figure 2
Specific (a) L. infantum and (b) rKMP11 antibody levels in sick dogs (Group 2) classified based on LeishVet clinical staging.
Figure 3
Figure 3
Specific ex vivo IFN-γ concentration after L. infantum, rKMP11 and ConA stimulation in apparently healthy non-infected dogs from a low endemicity area, Asturias, Spain (Group 1), sick dogs from Catalonia, Spain (Group 2), apparently healthy Ibizan hounds from a high endemicity area, Mallorca, Spain (Group 3) and apparently healthy dogs from a high endemicity area, Bari, Italy (Group 4).
See this image and copyright information in PMC

References

    1. Noli C., Saridomichelakis M.N. An update on the diagnosis and treatment of canine leishmaniosis caused by Leishmania infantum (syn. L. chagasi). Vet. J. 2014;202:425–435. doi: 10.1016/j.tvjl.2014.09.002. - DOI - PubMed
    1. Ready P.D. Leishmaniasis emergence in Europe. Eurosurveillance. 2010;15:1–11. doi: 10.2807/ese.15.10.19505-en. - DOI - PubMed
    1. Velez R., Ballart C., Domenech E., Abras A., Fernández-Arévalo A., Gómez S.A., Tebar S., Muñoz C., Cairó J., Gállego M. Seroprevalence of canine Leishmania infantum infection in the Mediterranean region and identification of risk factors: The example of North-Eastern and Pyrenean areas of Spain. Prev. Vet. Med. 2018;162:67–75. doi: 10.1016/j.prevetmed.2018.10.015. - DOI - PubMed
    1. Tabbabi A. Review of Leishmaniasis in the Middle East and North Africa. Afr Health Sci. 2019;19:1329–1337. doi: 10.4314/ahs.v19i1.4. - DOI - PMC - PubMed
    1. Dantas-Torres F. Canine leishmaniosis in South America. Parasit. Vectors. 2009;8:1–8. doi: 10.1186/1756-3305-2-S1-S1. - DOI - PMC - PubMed

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