Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2408:85-93.
doi: 10.1007/978-1-0716-1875-2_5.

Barley Stripe Mosaic Virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes in Wheat and Barley

Harvinder Bennypaul  1 , Upinder S Gill  2
Affiliations

Barley Stripe Mosaic Virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes in Wheat and Barley

Harvinder Bennypaul et al. Methods Mol Biol. 2022.

Abstract

Virus-induced gene silencing (VIGS) is an efficient method for functional characterization of genes in monocot and dicot plants via transient silencing of gene(s) of interest. Among various virus vectors, Barley stripe mosaic virus (BSMV) is established as a vector of choice to silence genes in wheat and barley. BSMV is a single-stranded positive-sense RNA virus with a tripartite genome consisting of α, β, and γ RNAs. BSMV-based VIGS has been used to silence both abiotic and biotic stress response genes in various growth stages of plants. Here we describe an efficient and effective protocol to successfully silence wheat and barley genes expressing in various tissues using this approach.

Keywords: Barley; Barley stripe mosaic virus; VIGS; Virus-induced gene silencing; Wheat.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Vance V, Vaucheret H (2001) RNA silencing in plants - defense and counterdefense. Science 292:2277–2280 - PubMed
    1. Robertson D (2004) VIGS vectors for gene silencing: many targets, many tools. Annu Rev Plant Biol 55(1):495–519 - PubMed
    1. Senthil-Kumar M, Mysore KS (2011) New dimensions for VIGS in plant functional genomics. Trends Plant Sci 16(12):656–665 - PubMed
    1. Holzberg S, Brosio P, Gross C, Pogue GP (2002) Barley stripe mosaic virus-induced gene silencing in a monocot plant. Plant J 30(3):315–327 - PubMed
    1. Palomar MK, Brakke MK, Jackson AO (1977) Base sequence homology in the RNAs of barley stripe mosaic virus. Virology 77(2):471–480 - PubMed

Supplementary concepts

LinkOut - more resources