Optimization of Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing (VIGS) in Tomato
- PMID: 35325421
- DOI: 10.1007/978-1-0716-1875-2_9
Optimization of Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing (VIGS) in Tomato
Abstract
Unveiling of full genome sequence of tomato demands significant advances of tomato functional genomics. Virus-induced gene silencing (VIGS) is a well explored functional genomics tool in plant biology that exploits post transcriptional gene silencing to downregulate a desired gene. Although VIGS provides an easy and highly efficient platform to study plant gene function through reverse genetics approach, currently VIGS is more efficient in model plants like Nicotiana benthamiana, which further justifies the urgent need of a highly efficient, reliable, and reproducible VIGS protocol in crop plants such as tomato. In this chapter, we have detailed an optimized Tobacco rattle virus (TRV)-based VIGS protocol in tomato.
Keywords: Agrobacterium; Functional genomics; PDS; PTGS; TRV; Tomato; VIGS.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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References
-
- Sahu PP, Puranik S, Khan M, Prasad M (2012) Recent advances in tomato functional genomics: utilization of VIGS. Protoplasma 249(4):1017–1027. https://doi.org/10.1007/s00709-012-0421-7 - DOI - PubMed
-
- Senthil-Kumar M, Mysore KS (2011) New dimensions for VIGS in plant functional genomics. Trends Plant Sci 16(12):656–665. https://doi.org/10.1016/j.tplants.2011.08.006 - DOI - PubMed
-
- Becker A, Lange M (2010) VIGS – genomics goes functional. Trends Plant Sci 15(1):1–4. https://doi.org/10.1016/j.tplants.2009.09.002 - DOI
-
- Liu Y, Schiff M, Marathe R, Dinesh-Kumar SP (2002) Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus. Plant J 30(4):415–429. https://doi.org/10.1046/j.1365-313X.2002.01297.x - DOI - PubMed
-
- Senthil-Kumar M, Mysore KS (2011) Virus-induced gene silencing can persist for more than 2 years and also be transmitted to progeny seedlings in Nicotiana benthamiana and tomato. Plant Biotechnol J 9(7):797–806. https://doi.org/10.1111/j.1467-7652.2011.00589.x - DOI - PubMed
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