Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2408:133-145.
doi: 10.1007/978-1-0716-1875-2_9.

Optimization of Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing (VIGS) in Tomato

Ashish Kumar Singh  1 , Dibyendu Ghosh  1 , Supriya Chakraborty  2
Affiliations

Optimization of Tobacco Rattle Virus (TRV)-Based Virus-Induced Gene Silencing (VIGS) in Tomato

Ashish Kumar Singh et al. Methods Mol Biol. 2022.

Abstract

Unveiling of full genome sequence of tomato demands significant advances of tomato functional genomics. Virus-induced gene silencing (VIGS) is a well explored functional genomics tool in plant biology that exploits post transcriptional gene silencing to downregulate a desired gene. Although VIGS provides an easy and highly efficient platform to study plant gene function through reverse genetics approach, currently VIGS is more efficient in model plants like Nicotiana benthamiana, which further justifies the urgent need of a highly efficient, reliable, and reproducible VIGS protocol in crop plants such as tomato. In this chapter, we have detailed an optimized Tobacco rattle virus (TRV)-based VIGS protocol in tomato.

Keywords: Agrobacterium; Functional genomics; PDS; PTGS; TRV; Tomato; VIGS.

PubMed Disclaimer

References

    1. Sahu PP, Puranik S, Khan M, Prasad M (2012) Recent advances in tomato functional genomics: utilization of VIGS. Protoplasma 249(4):1017–1027. https://doi.org/10.1007/s00709-012-0421-7 - DOI - PubMed
    1. Senthil-Kumar M, Mysore KS (2011) New dimensions for VIGS in plant functional genomics. Trends Plant Sci 16(12):656–665. https://doi.org/10.1016/j.tplants.2011.08.006 - DOI - PubMed
    1. Becker A, Lange M (2010) VIGS – genomics goes functional. Trends Plant Sci 15(1):1–4. https://doi.org/10.1016/j.tplants.2009.09.002 - DOI
    1. Liu Y, Schiff M, Marathe R, Dinesh-Kumar SP (2002) Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus. Plant J 30(4):415–429. https://doi.org/10.1046/j.1365-313X.2002.01297.x - DOI - PubMed
    1. Senthil-Kumar M, Mysore KS (2011) Virus-induced gene silencing can persist for more than 2 years and also be transmitted to progeny seedlings in Nicotiana benthamiana and tomato. Plant Biotechnol J 9(7):797–806. https://doi.org/10.1111/j.1467-7652.2011.00589.x - DOI - PubMed

Publication types

Supplementary concepts

LinkOut - more resources