Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2408:181-189.
doi: 10.1007/978-1-0716-1875-2_12.

High-Throughput Analysis of Gene Function under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants

Ramegowda Yamunarani  1 , Venkategowda Ramegowda  2 , Muthappa Senthil-Kumar  3 , Kirankumar S Mysore  4
Affiliations

High-Throughput Analysis of Gene Function under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants

Ramegowda Yamunarani et al. Methods Mol Biol. 2022.

Abstract

The high throughputness and affordability of "omics" technologies is leading to the identification of a large number of abiotic stress genes, with many of them responsive to multiple stresses. In vivo functional characterization of these genes under multiple stresses is challenging but essential to develop resilient crops for the changing climate. Here we describe a high-throughput Virus-Induced Gene Silencing-based methodology for functional analysis of genes under multiple abiotic stresses using leaf disks. Leaves with maximal silencing, which is localized to only a few leaves and to a short period, can be effectively used for multiple stress imposition and stress affect quantification.

Keywords: Leaf disks; Multiple abiotic stresses; Virus-Induced Gene Silencing.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Bray EA, Bailey-Serres J, Weretilnyk E, Buchannan B, Jones R (2000) Responses to abiotic stresses. In: Biochemistry and molecular biology of plants. American Society of Plant Biologists, Rockville, MD, pp 149–158
    1. Xiong L, Schumaker KS, Zhu JK (2002) Cell signaling during cold, drought, and salt stress. Plant Cell 14(Suppl):S165–S183 - PubMed - PMC
    1. Haak DC, Fukao T, Grene R, Hua Z, Ivanov R, Perrella G et al (2017) Multilevel regulation of abiotic stress responses in plants. Front Plant Sci 8:1564 - PubMed - PMC
    1. Shinozaki K, Yamaguchi-Shinozaki K (2007) Gene networks involved in drought stress response and tolerance. J Exp Bot 58(2):221–227 - PubMed
    1. Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K (2014) The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold and heat. Front Plant Sci 5:170 - PubMed - PMC

Publication types

LinkOut - more resources