Changes in miR-124-1, miR-212, miR-132, miR-134, and miR-155 Expression Patterns after 7,12-Dimethylbenz(a)anthracene Treatment in CBA/Ca Mice

doi:10.3390/cells11061020

. 2022 Mar 17;11(6):1020.

doi: 10.3390/cells11061020.

Changes in miR-124-1, miR-212, miR-132, miR-134, and miR-155 Expression Patterns after 7,12-Dimethylbenz(a)anthracene Treatment in CBA/Ca Mice

Andras Tomesz^{1

2}, Laszlo Szabo^{1

2}, Richard Molnar^{1

2}, Arpad Deutsch¹, Richard Darago¹, Bence L Raposa¹, Nowrasteh Ghodratollah², Timea Varjas², Balazs Nemeth², Zsuzsanna Orsos², Eva Pozsgai², Jozsef L Szentpeteri³, Ferenc Budan^{3

4}, Istvan Kiss²

Affiliations

¹ Doctoral School of Health Sciences, Faculty of Health Sciences, University of Pécs, 7624 Pécs, Hungary.
² Department of Public Health Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.
³ Institute of Transdisciplinary Discoveries, Medical School, University of Pécs, 7624 Pécs, Hungary.
⁴ Institute of Physiology, Medical School, University of Pécs, 7624 Pécs, Hungary.

PMID: 35326471
PMCID: PMC8947631
DOI: 10.3390/cells11061020

Changes in miR-124-1, miR-212, miR-132, miR-134, and miR-155 Expression Patterns after 7,12-Dimethylbenz(a)anthracene Treatment in CBA/Ca Mice

Andras Tomesz et al. Cells. 2022.

. 2022 Mar 17;11(6):1020.

doi: 10.3390/cells11061020.

Authors

Affiliations

¹ Doctoral School of Health Sciences, Faculty of Health Sciences, University of Pécs, 7624 Pécs, Hungary.
² Department of Public Health Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary.
³ Institute of Transdisciplinary Discoveries, Medical School, University of Pécs, 7624 Pécs, Hungary.
⁴ Institute of Physiology, Medical School, University of Pécs, 7624 Pécs, Hungary.

PMID: 35326471
PMCID: PMC8947631
DOI: 10.3390/cells11061020

Abstract

Specific gene and miRNA expression patterns are potential early biomarkers of harmful environmental carcinogen exposures. The aim of our research was to develop an assay panel by using several miRNAs for the rapid screening of potential carcinogens. The expression changes of miR-124-1, miR-212, miR-132, miR-134, and miR-155 were examined in the spleen, liver, and kidneys of CBA/Ca mice, following the 20 mg/bwkg intraperitoneal 7,12-dimethylbenz(a)anthracene (DMBA) treatment. After 24 h RNA was isolated, the miRNA expressions were analyzed by a real-time polymerase chain reaction and compared to a non-treated control. DMBA induced significant changes in the expression of miR-134, miR-132, and miR-124-1 in all examined organs in female mice. Thus, miR-134, miR-132, and miR-124-1 were found to be suitable biomarkers for the rapid screening of potential chemical carcinogens and presumably to monitor the protective effects of chemopreventive agents.

Keywords: 7,12-dimethylbenz(a)anthracene; carcinogen; miR-124-1; miR-132; miR-134; miR-155; miR-212; miRNA.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

**Figure 1**
Expression results of miR-134, miR-132, miR-124-1, miR-212, and miR-155 in the liver of (A) female and (B) male CBA/Ca mice 24 h after DMBA treatment, compared to non-treated mice (100%) (* p < 0.05; *** p < 0.001).

**Figure 2**
Expression results of miR-134, miR-132, miR-124-1, miR-212, and miR-155 in the spleen of (A) female and (B) male CBA/Ca mice 24 h after DMBA treatment, compared to non-treated mice (100%) (*** p < 0.001).

**Figure 3**
Expression results of miR-134, miR-132, miR-124-1, miR-212, and miR-155 in the kidneys of (A) female and (B) male CBA/Ca mice 24 h after DMBA treatment, compared to non-treated mice (100%) (* p < 0.05; *** p < 0.001).

**Figure 4**
Expression results of miR-134 and miR-124-1 in the liver, kidneys, and spleen of CBA/Ca mice 24 h after DMBA treatment, compared to non-treated mice (100%) (* p < 0.05; *** p < 0.001).

**Figure 5**
Summary of the affected relevant signaling pathways and the role of the expression changes of the investigated miRNAs (miR-134, miR-132, miR-124-1, miR-212, and miR-155) 24 h after DMBA treatment.

See this image and copyright information in PMC

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[1] Sung H., Ferlay J., Siegel R.L., Laversanne M., Soerjomataram I., Jemal A., Bray F. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J. Clin. 2021;71:209–249. doi: 10.3322/caac.21660. - DOI - PubMed

[2] Sung H., Ferlay J., Siegel R.L., Laversanne M., Soerjomataram I., Jemal A., Bray F. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J. Clin. 2021;71:209–249. doi: 10.3322/caac.21660. - DOI - PubMed

[3] Danaei G., Vander Hoorn S., Lopez A.D., Murray C.J., Ezzati M. Comparative Risk Assessment Collaborating Group (Cancers). Causes of cancer in the world: Comparative risk assessment of nine behavioural and environmental risk factors. Lancet. 2005;366:1784–1793. doi: 10.1016/S0140-6736(05)67725-2. - DOI - PubMed

[4] Danaei G., Vander Hoorn S., Lopez A.D., Murray C.J., Ezzati M. Comparative Risk Assessment Collaborating Group (Cancers). Causes of cancer in the world: Comparative risk assessment of nine behavioural and environmental risk factors. Lancet. 2005;366:1784–1793. doi: 10.1016/S0140-6736(05)67725-2. - DOI - PubMed

[5] Frenk S., Houseley J. Gene expression hallmarks of cellular ageing. Biogerontology. 2018;19:547–566. doi: 10.1007/s10522-018-9750-z. - DOI - PMC - PubMed

[6] Frenk S., Houseley J. Gene expression hallmarks of cellular ageing. Biogerontology. 2018;19:547–566. doi: 10.1007/s10522-018-9750-z. - DOI - PMC - PubMed

[7] Kwa M., Makris A., Esteva F.J. Clinical utility of gene-expression signatures in early stage breast cancer. Nat. Rev. Clin. Oncol. 2017;14:595–610. doi: 10.1038/nrclinonc.2017.74. - DOI - PubMed

[8] Kwa M., Makris A., Esteva F.J. Clinical utility of gene-expression signatures in early stage breast cancer. Nat. Rev. Clin. Oncol. 2017;14:595–610. doi: 10.1038/nrclinonc.2017.74. - DOI - PubMed

[9] Yang Z.-Y., Liu X.-Y., Shu J., Zhang H., Ren Y.-Q., Xu Z.-B., Liang Y. Multi-view based integrative analysis of gene expression data for identifying biomarkers. Sci. Rep. 2019;9:1775–1781. doi: 10.1038/s41598-019-49967-4. - DOI - PMC - PubMed

[10] Yang Z.-Y., Liu X.-Y., Shu J., Zhang H., Ren Y.-Q., Xu Z.-B., Liang Y. Multi-view based integrative analysis of gene expression data for identifying biomarkers. Sci. Rep. 2019;9:1775–1781. doi: 10.1038/s41598-019-49967-4. - DOI - PMC - PubMed

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Changes in miR-124-1, miR-212, miR-132, miR-134, and miR-155 Expression Patterns after 7,12-Dimethylbenz(a)anthracene Treatment in CBA/Ca Mice

Affiliations

Changes in miR-124-1, miR-212, miR-132, miR-134, and miR-155 Expression Patterns after 7,12-Dimethylbenz(a)anthracene Treatment in CBA/Ca Mice

Authors

Affiliations

Abstract

Conflict of interest statement

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Abstract

Conflict of interest statement

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