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. 2022 Mar 10;12(6):700.
doi: 10.3390/ani12060700.

Wound Retractor Laparoscopic Port System for Laparoscopic Ovariectomy in Panthera leo

Luca Lacitignola  1 , Pietro Laricchiuta  2 , Annarita Imperante  3 , Claudia Acquafredda  3 , Marzia Stabile  3 , Annalaura Scardia  3 , Marta Guadalupi  3 , Caterina Vicenti  3 , Alberto Crovace  4 , Francesco Staffieri  1
Affiliations

Wound Retractor Laparoscopic Port System for Laparoscopic Ovariectomy in Panthera leo

Luca Lacitignola et al. Animals (Basel). .

Abstract

The aim of this study was to assess the feasibility and intraoperative complications of performing ovariectomies in African lionesses (Panthera leo) using a wound retractor laparoscopic platform. Six lionesses (n = 6) were included. The surgical procedures were carried out through three portals, with a retractor platform positioned at the umbilical port and cannulas placed 3-4 cm from the cranial and caudal regions to the device at the level of the midline. An ovariectomy was performed with a vessel-sealing device. We evaluated the surgery time and the intraoperative and early postoperative complications. The mean weight was 172.83 kg. The total surgery time was 49.33 min. The installation step took a mean of 10.33 min to complete. The mean ovariectomy time was 20 min. Controlled bleeding was observed at the tip of the uterine horn in two cases due to excessive tissue thickness. The retrieval of dissected ovarian tissue and annexes was easily performed. No other complications were observed. The use of the laparoscopic platform during three-portal surgeries for laparoscopic ovariectomy in adult overweight lionesses is feasible and without intraoperative problems. The retractor meant that there were no entry-related issues due to the 25 mm mini-laparotomy. It also made it simpler to extract thick ovaries and promptly re-establish the pneumoperitoneum.

Keywords: Panthera leo; laparoscopy; lionesses; ovariectomy; wound retractor device.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Images of wound retractor laparoscopic platform employed: (a) cap; (a1) instrumental port; (a2) CO2 inlet plug; (b) retractor; (b1) outer ring; (b2) polyurethan cylindrical sleeve; (b3) inner ring; (b4) removal tag.
Figure 2
Figure 2
Installation of WRD laparoscopic platform. The WRD was inserted at midline ca. 1 cm caudal to the umbilical scar. Cranial is on the right. (A) Inner ring placement through 25 mm mini-laparotomy. The inner ring was squeezed to allow entrance in the abdominal cavity. (B) After the proper installation of the inner ring, the outer ring was adjusted for correct wound retraction and stable fixation to the abdominal wall. (C) Complete WRD installation and wound retraction. (D) The cap was mounted on the outer ring for tight portal sealing, CO2 gas tube connection, and instrument introduction.
Figure 3
Figure 3
Complete installation of the three portals. Cranial is on the right. The cranial and caudal 12 mm cannulas were placed in the midline 3–4 cm from WRD border.
Figure 4
Figure 4
Representative laparoscopic images of right ovariectomy. Caudal is on the left. (A) Ovary inspection. (B) The ovary was grasped and suspended for ovarian vessel bound visualization. (C) The ovarian vessel bound was clamped with the vessel-sealing device axially for complete dissection. (D) The mesovarium was dissected with the vessel-sealing device. (E) The uterine horn was clamped and coagulated at the level of proper ovarian ligament with vessel-sealing device. Many coagulation cycles were applied to complete dissection. (F) Complete Ovary dissection.
Figure 5
Figure 5
Portals sutured. Cranial is on the right.
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