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Comparative Study
. 1986 Sep;52(3):430-3.
doi: 10.1128/aem.52.3.430-433.1986.

Practical direct plaque assay for coliphages in 100-ml samples of drinking water

W O Grabow, P Coubrough
Comparative Study

Practical direct plaque assay for coliphages in 100-ml samples of drinking water

W O Grabow et al. Appl Environ Microbiol. 1986 Sep.

Abstract

A practical single-agar-layer plaque assay for the direct detection of coliphages in 100-ml samples of water was designed and evaluated. With this assay a 100-ml sample of water, an agar medium containing divalent cations, and the host Escherichia coli C (ATCC 13706) were mixed in a single container, and the mixture was plated on 10 14-cm-diameter petri dishes. It was more sensitive, reliable, and accurate than various other methods and proved rapid, simple, and economic.

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