Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar 12;11(6):1563.
doi: 10.3390/jcm11061563.

A Three-Protein Panel to Support the Diagnosis of Sepsis in Children

Francisco J Pilar-Orive  1   2 , Itziar Astigarraga  3   4   5 , Mikel Azkargorta  6 , Felix Elortza  6 , Susana Garcia-Obregon  3   7
Affiliations

A Three-Protein Panel to Support the Diagnosis of Sepsis in Children

Francisco J Pilar-Orive et al. J Clin Med. .

Abstract

Sepsis is a syndrome without a standard validated diagnostic test. Early recognition is crucial. Serum proteome analysis in children with sepsis may identify new biomarkers. This study aimed to find suitable blood biomarkers for an early diagnosis of sepsis. An analytical observational case-control study was carried out in a single center. Children admitted to a Pediatric Intensive Care Unit with clinical diagnosed sepsis were eligible for study. A proteomic analysis conducted by mass spectrometry was performed. Forty patients with sepsis and 24 healthy donors were recruited. Proteomics results revealed 44 proteins differentially expressed between patients and healthy controls. Six proteins were selected to be validated: lactoferrin, serum amyloid-A1 (SAA-1), complement factor B, leucine-rich alpha-2 glycoprotein (LRG1), soluble interleukin-2 alpha chain receptor (sCD25) and soluble haptoglobin−hemoglobin receptor. Our results showed that sCD25, SAA-1, and LRG1 had high levels of specificity and sensitivity, as well as an excellent area under the ROC curve (>0.9). Our study provides a serum proteomic analysis that identifies new diagnostic biomarkers in sepsis. SAA-1, sCD25 and LRG1 were able to separate septic from healthy donor, so they could be used together with other clinical and analytical features to improve sepsis diagnosis in children.

Keywords: biomarkers; children; mass spectrometry analysis; proteome; sepsis; septic shock.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Significantly deregulated proteins among different groups.
Figure 2
Figure 2
Flow chart performed for deciding validation of proteins.
Figure 3
Figure 3
Area under the receiver operating characteristic (AUROC) and box plot for SAA-1, sCD25 and LRG1. Among these proteins studied as potential biomarkers, SAA-1 (AUROC 0.978; 95% CI: 0.946–1), sCD25 (0.97; 95% CI: 0.92–1) and LRG1 (0.93; 95% CI: 0.86–1), showed an area under the ROC curve greater than 0.9.
See this image and copyright information in PMC

References

    1. Singer M., Deutschman C.S., Seymour C.W., Shankar-Hari M., Annane D., Bauer M., Bellomo R., Bernard G.R., Chiche J.-D., Coopersmith C.M., et al. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3) JAMA. 2016;315:801–810. doi: 10.1001/jama.2016.0287. - DOI - PMC - PubMed
    1. Matics T.J., Sanchez-Pinto L.N. Adaptation and Validation of a Pediatric Sequential Organ Failure Assessment Score and Evaluation of the Sepsis-3 Definitions in Critically Ill Children. JAMA Pediatr. 2017;171:e172352. doi: 10.1001/jamapediatrics.2017.2352. - DOI - PMC - PubMed
    1. Goldstein B., Giroir B., Randolph A. International pediatric sepsis consensus conference: Definitions for sepsis and organ dysfunction in pediatrics. Pediatr. Crit. Care Med. 2005;6:2–8. doi: 10.1097/01.PCC.0000149131.72248.E6. - DOI - PubMed
    1. Weiss S.L., Peters M.J., Alhazzani W., Agus M.S.D., Flori H.R., Inwald D.P., Nadel S., Schlapbach L.J., Tasker R.C., Argent A.C., et al. Surviving Sepsis Campaign International Guidelines for the Management of Septic Shock and Sepsis-Associated Organ Dysfunction in Children. Pediatr. Crit. Care Med. 2020;21:e52–e106. doi: 10.1097/PCC.0000000000002198. - DOI - PubMed
    1. Fleischmann-Struzek C., Goldfarb D., Schlattmann P., Schlapbach L.J., Reinhart K., Kissoon N. The global burden of paediatric and neonatal sepsis: A systematic review. Lancet Respir. Med. 2018;6:223–230. doi: 10.1016/S2213-2600(18)30063-8. - DOI - PubMed

LinkOut - more resources