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. 2022 Mar 18;12(3):451.
doi: 10.3390/life12030451.

Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp

Wadzani Palnam Dauda  1 , Peter Abraham  2 , Elkanah Glen  3 , Charles Oluwaseun Adetunji  4 , Shakira Ghazanfar  5 , Shafaqat Ali  6   7 , Majid Al-Zahrani  8 , Mawuli Kwamla Azameti  9 , Sheik Emmanuel Laykay Alao  10 , Afiniki Bawa Zarafi  10 , Maryam Peter Abraham  2 , Hannatu Musa  11
Affiliations

Robust Profiling of Cytochrome P450s (P450ome) in Notable Aspergillus spp

Wadzani Palnam Dauda et al. Life (Basel). .

Abstract

Cytochrome P450s (P450ome) constitute an extended superfamily group of heme-thiolate enzymes identified in all biological domains. P450omes play a critical role in the oxidation of steroids and fatty acids, xenobiotic degradation of hydrophobic compounds, biosynthesis of hormones, and primary and secondary metabolism in organisms. Aspergillus species are among the most economically important fungal organisms in human medicine, industry, and agriculture worldwide. Exploring insight on the genome-wide annotations of cytochrome P450s in Aspergillus species is necessary for their biosynthetic applications. In this present study, we report the identification of 306 cytochrome P450s and their robust profiling in eight notable Aspergillus species (A. carbonarius, A. clavatus, A. flavus, A. fumigatus, A. nidulans, A. niger, A. oryzae, and A. terreus). Based on the evolutionary relationship, the Aspergillus P450s families clustered into 15 clades, with clades V, I, and XIII recording higher percentages (17.3%, 15.00%, and 14.71%, respectively) of Cyp families. Cyps were classified into 120 families 64 clans, and their putative functions were also elucidated. P450s were predicted to be located in 13 subcellular components, but the endoplasm reticulum was the dominant location across the eight Aspergillus species. Cyps genes of Aspergillus species were associated with seven secondary metabolism-related gene clusters. Elucidating the genome-wide annotations of P450s enzymes in Aspergillus species will form vital potential biotechnological tools that could be harnessed for industrial, pharmaceutical, and agricultural use.

Keywords: Aspergillus spp.; P450s; fungi; phylogeny; secondary metabolite.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Various cytochrome P450 families predicted in eight Aspergillus species. NB: Green-colored boxes indicate the predicted CYP families in each Aspergillus species.
Figure 2
Figure 2
Evolutionary relationships of cytochrome P450 proteins among eight Aspergillus species (A. carbonarius, A. clavatus, A. flavus, A. fumigatus, A. nidulans, A. niger, A. oryzae, and A. terreus). Phylogenetic tree was inferred using the minimum evolution method with MEGAX software. Each phylogenetic group (I–XV) is indicated by a specific color.
Figure 3
Figure 3
Prediction of subcellular localization analysis of protein genes of eight Aspergillus species.
Figure 4
Figure 4
Secondary Metabolism-Related Gene Clusters for the Eight Aspergillus Species.
See this image and copyright information in PMC

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