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. 2022 Mar 7:10:855136.
doi: 10.3389/fbioe.2022.855136. eCollection 2022.

Investigation of Antimicrobial and Cytotoxic Properties and Specification of Silver Nanoparticles (AgNPs) Derived From Cicer arietinum L. Green Leaf Extract

Ayşe Baran  1 , Mehmet Fırat Baran  2   3 , Cumali Keskin  2   3 , Abdulkerim Hatipoğlu  4 , Ömer Yavuz  5   6 , Sevgi İrtegün Kandemir  7 , Mehmet Tevfik Adican  8   9 , Rovshan Khalilov  9   10   11 , Afat Mammadova  12 , Elham Ahmadian  13 , Gvozden Rosić  14 , Dragica Selakovic  14 , Aziz Eftekhari  15   16
Affiliations

Investigation of Antimicrobial and Cytotoxic Properties and Specification of Silver Nanoparticles (AgNPs) Derived From Cicer arietinum L. Green Leaf Extract

Ayşe Baran et al. Front Bioeng Biotechnol. .

Abstract

Using biological materials to synthesize metallic nanoparticles has become a frequently preferred method by researchers. This synthesis method is both fast and inexpensive. In this study, an aqueous extract obtained from chickpea (Cicer arietinum L.) (CA) leaves was used in order to synthesize silver nanoparticles (AgNPs). For specification of the synthesized AgNPs, UV-vis spectrophotometer, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), electron dispersive X-ray (EDX), and zeta potential (ZP) analyses data were used. Biologically synthesized AgNPs demonstrated a maximum surface plasmon resonance of 417.47 nm after 3 h. With the powder XRD model, the mean crystallite dimension of nanoparticles was determined as 12.17 mm with a cubic structure. According to the TEM results, the dimensions of the obtained silver nanoparticles were found to be 6.11-9.66 nm. The ZP of the electric charge on the surface of AgNPs was measured as -19.6 mV. The inhibition effect of AgNPs on food pathogen strains and yeast was determined with the minimum inhibition concentration (MIC) method. AgNPs demonstrated highly effective inhibition at low concentrations especially against the growth of B. subtilis (0.0625) and S. aureus (0.125) strains. The cytotoxic effects of silver nanoparticles on cancerous cell lines (CaCo-2, U118, Sk-ov-3) and healthy cell lines (HDF) were revealed. Despite the increase of AgNPs used against cancerous and healthy cell lines, no significant decrease in the percentage of viability was detected.

Keywords: SEM-EDX; cytotoxic activity; food pathogens; green synthesis; nanomaterials; nanomedicine.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Graphical illustration of the study.
FIGURE 2
FIGURE 2
UV-vis absorption spectra of CA-AgNPs.
FIGURE 3
FIGURE 3
(A–D) SEM images of CA-AgNPs in different scanning areas.
FIGURE 4
FIGURE 4
TEM results of CA-AgNPs.
FIGURE 5
FIGURE 5
(A) FT-IR spectra data of CA leaf extract. (B) FT-IR spectra data of synthesized CA-AgNPs.
FIGURE 6
FIGURE 6
Elemental composition of AgNPs with EDX analysis.
FIGURE 7
FIGURE 7
XRD patterns of biosynthesized AgNPs.
FIGURE 8
FIGURE 8
Zeta potential data of AgNPs.
FIGURE 9
FIGURE 9
Evaluation of the percentage viability rates as a result of the cytotoxic effect of AgNPs 2 days after combining them with CaCo-2, U118, HDF, and Sk-ov-3 cell lines.
See this image and copyright information in PMC

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