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. 2022 Jun 9;59(6):2102802.
doi: 10.1183/13993003.02802-2021. Print 2022 Jun.

Shunt-type plexiform lesions identified in the Sugen5416/hypoxia rat model of pulmonary arterial hypertension using synchrotron-based phase-contrast micro-CT

Oscar van der Have  1 , Christian Westöö  2 , Filip Ahrné  3 , Xuefei Tian  4   5 , Kenzo Ichimura  4   5 , Till Dreier  6 , Christian Norvik  2 , Maya E Kumar  5   7 , Edda Spiekerkoetter  4   5   8 , Karin Tran-Lundmark  2   3   9
Affiliations

Shunt-type plexiform lesions identified in the Sugen5416/hypoxia rat model of pulmonary arterial hypertension using synchrotron-based phase-contrast micro-CT

Oscar van der Have et al. Eur Respir J. .

Abstract

Human like plexiform lesions identified in the prolonged Sugen5416/hypoxia rat model, visualised by synchrotron tomography imaging https://bit.ly/3KQvDHg

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Conflict of interest statement

Conflict of interest: C. Westöö declares a regional salary grant for medical resident researchers from ALF Forskningsutrymme för ST-läkare. T. Dreier declares an industrial PhD salary from Excillum AB, and payment to their institution from the Swedish Foundation for Strategic Research (SSF) in connection with the present manuscript. M.E. Kumar declares American Heart Association Scientist Development Grant AHA 16SDG30030006, Stanford Pediatrics startup funds 1246483-200-JHAJH, Stanford Spectrum Child Health Research Institute 1170805-100-GHEBG, Stanford Maternal and Child Health Research Institute Pilot Grant 1220318-108-JHACT, Vera Moulton Wall Center for Pulmonary Vascular Research grant 1144545-401-GHDCK and a Bravo Family Endowed Faculty Scholarship, in connection with the present manuscript. E. Spiekerkoetter declares that they have a research scholarship from Vera Moulton Wall Center for Pulmonary Vascular Disease, in connection with the present manuscript; and that they have received grants from the National Institutes of Health (National Heart, Lung and Blood Institute grants R01HL158868 and R01 HL128734) and Department of Defense (grants PR161256 and PR 181774) in the 36 months prior to manuscript submission; and that they receive royalties from Stanford University for Method of use patent “Use of FK506 for the treatment of Pulmonary Arterial Hypertension”; that they have a provisional US patent for “Enzastaurin and Fragile Histidine Trial (FHIT) Increasing Agents for the Treatment of Pulmonary Hypertension”; and that they are chair of the American Heart Association 3CPR Early Career Committee, and a member of the Stanford University Institutional Review Board. K. Tran-Lundmark declares funding from the Swedish Heart-Lung Foundation, the Crafoord Foundation, the Swedish Society of Medicine, the Knut and Alice Wallenberg Foundation and the Skåne County Council, in connection with the present manuscript; as well as roles as American Thoracic Society (ATS) Pulmonary Circulation Program Committee member (unpaid); American Heart Association (AHA) 3CPR Early Career Committee (unpaid) and Association for European Paediatric and Congenital Cardiology (AEPC) Councillor in the Working Group for Pulmonary Hypertension, Heart Failure and Transplantation (unpaid). All other authors declare no competing interests.

Figures

FIGURE 1
FIGURE 1
Three-dimensional reconstructions of the four types of plexiform lesions in the rat model. All four types of plexiform lesions previously described in human pulmonary arterial hypertension were identified (1–4) and the injected radiopaque dye was used to three-dimensionally reconstruct the vascular lumen of the different lesions. White asterisks mark the three-dimensional reconstructed plexiform lesions and, in insets 1–4, the same lesions in two-dimensional cross sections, as they would have been seen histologically. Type 1 lesions derive from monopodial branches/supernumerary arteries (black arrowhead), with connections to the vasa vasorum (white arrowheads). Type 2 lesion are best described as tortuous transformations of IBAs (black arrowhead), connected to peribronchial vessels (white arrowheads). Type 3 are found at abrupt ends of distal, intra-acinar, pulmonary arteries/arterioles and type 4 lesions are blocked pulmonary arteries/arterioles with re-canalisation. All four types of plexiform lesions are also illustrated in multi-dimensional video format (supplementary videos 1–4). PA: pulmonary artery; A: airway.
See this image and copyright information in PMC

References

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