Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Feb 23;10(3):491.
doi: 10.3390/microorganisms10030491.

The Use of Long-Read Sequencing Technologies in Infection Control: Horizontal Transfer of a blaCTX-M-27 Containing lncFII Plasmid in a Patient Screening Sample

Vincent van Almsick  1   2 , Franziska Schuler  3 , Alexander Mellmann  1 , Vera Schwierzeck  1
Affiliations

The Use of Long-Read Sequencing Technologies in Infection Control: Horizontal Transfer of a blaCTX-M-27 Containing lncFII Plasmid in a Patient Screening Sample

Vincent van Almsick et al. Microorganisms. .

Abstract

Plasmid transfer is one important mechanism how antimicrobial resistance can spread between different species, contributing to the rise of multidrug resistant bacteria (MDRB) worldwide. Here were present whole genome sequencing (WGS) data of two MDRB isolates, an Escherichia coli and a Klebsiella quasipneumoniae, which were isolated from a single patient. Detailed analysis of long-read sequencing data identified an identical F2:A-:B- lncFII plasmid containing blaCTX-M-27 in both isolates, suggesting horizontal plasmid exchange between the two species. As the plasmid of the E. coli strain carried multiple copies of the resistance cassette, the genomic data correlated with the increased antimicrobial resistance (AMR) detected for this isolate. Our case report demonstrates how long-read sequencing data of MDRB can be used to investigate the role of plasmid mediate resistance in the healthcare setting and explain resistance phenotypes.

Keywords: blaCTX-M-27; long-read sequencing; plasmid transfer.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
Comparison of the K. quasipneumoniae plasmid and the reference plasmid p3347558_4 using BRIG. The outer Ring shows the annotated sequences by dfast: The AMG cassette is colored in blue, all other proteins are colored in red.
Figure 2
Figure 2
(a): progessiveMauve alignment of the AMG-cassettes: R = AMG-cassette of the K. quasipneumoniae plasmid; R1-3 = AMG-cassettes of the E. coli plasmid; FtsI = peptidoglycan glycosyltransferase FtsI; LAP-2 = blaLAP-2; hp = hypotetical protein; qnrS1 = quinolone resistance protein qnrS1; tsp. = transpoase; CTX-M-27 = blaCTX-M-27. IS91 & IS5 family tsp. lost in R3 by frameshift because of insertions and deletions. (b): schematic blueprint of the two plasmids.
See this image and copyright information in PMC

References

    1. Laxminarayan R., Duse A., Wattal C., Zaidi A.K.M., Wertheim H.F.L., Sumpradit N., Vlieghe E., Hara G.L., Gould I.M., Goossens H., et al. Antibiotic resistance-the need for global solutions. Lancet Infect. Dis. 2013;13:1057–1098. doi: 10.1016/S1473-3099(13)70318-9. - DOI - PubMed
    1. Carattoli A. Plasmids and the spread of resistance. Int. J. Med. Microbiol. 2013;303:298–304. doi: 10.1016/j.ijmm.2013.02.001. - DOI - PubMed
    1. Novais A., Viana D., Baquero F., Martinez-Botas J., Canton R., Coque T.M. Contribution of IncFII and broad-host IncA/C and IncN plasmids to the local expansion and diversification of phylogroup B2 Escherichia coli ST131 clones carrying blaCTX-M-15 and qnrS1 genes. Antimicrob. Agents Chemother. 2012;56:2763–2766. doi: 10.1128/AAC.06001-11. - DOI - PMC - PubMed
    1. Patino-Navarrete R., Rosinski-Chupin I., Cabanel N., Zongo P.D., Hery M., Oueslati S., Girlich D., Dortet L., Bonnin R.A., Naas T., et al. Specificities and Commonalities of Carbapenemase-Producing Escherichia coli Isolated in France from 2012 to 2015. mSystems. 2022;7:e01169-21. doi: 10.1128/msystems.01169-21. - DOI - PMC - PubMed
    1. Zautner A.E., Bunk B., Pfeifer Y., Sproer C., Reichard U., Eiffert H., Scheithauer S., Gross U., Overmann J., Bohne W. Monitoring microevolution of OXA-48-producing Klebsiella pneumoniae ST147 in a hospital setting by SMRT sequencing. J. Antimicrob. Chemother. 2017;72:2737–2744. doi: 10.1093/jac/dkx216. - DOI - PubMed