Effects of light regime on circadian rhythmic behavior and reproductive parameters in native laying hens

Abstract

The paper aimed to study the effects of light regime on circadian rhythmic behavior and reproductive parameters in a native laying hen, Beijing You Chicken (BYC) during 22 to 30 wk. A total of 630 19-wk-old BYC female chicken were allocated to 6 light regime groups with 3 replicates per group and 35 birds per replicate, reared in individually lit floor pens with separate outdoor areas. A 2 × 3 factorial experiment (2 lighting patterns: continuous and intermittent lighting; 3 photoperiods: 16 h, 14 h, 12 h) was arranged, including 16L:8D for group 1; 12L:2D:4L:6D for group 2; 14L:10D for group 3; 10L:2D:4L:8D for group 4; 12L:12D for group 5, and 8L:4D:4L:8D for group 6, respectively. The circadian rhythmic behavior of the hens, including feeding, egg-laying and sleeping behaviors were observed by scan sampling and target sampling method for consecutive 3 d every other week during 22 to 30 wk. Infrared cameras were set outside each pen to record the rhythmic behaviors of the birds every other hour (6:00-7:00;8:00-9:00;10:00-11:00;12:00-13:00;14:00-15:00;16:00-17:00;18:00-19:00;20:00-21:00;22:00-23:00;0:00-1:00;2:00-3:00;4:00-5:00). Theovarian weight, oviduct weight, oviduct length, the number of large yellow follicles (LYF), and small yellow follicles (SYF) were measured at the end of 30 wk. The egg-laying rate during 22 to 30 wk was measured. The results showed that the highest feeding frequency was given by the 16L:8D, and the lowest given by the 12L:12D (P < 0.05). The average feeding duration was the longest in the 8L:4D:4L:8D at 6:00 to 8:00 (18.67 min/hen) (P < 0.05), and the shortest in the 8L:4D:4L:8D at 18:00 to 20:00 (3.75 min/hen) (P < 0.05). The highest egg-laying frequency was given by the 8L:4D:4L:8D (0.28), the lowest given by the 10L:2D:4L:8D (0.21) (P < 0.05). The shortest egg-laying duration occurred at 6:00 to 8:00, was given by the 16L:8D (8 min/hen), and the longest egg-laying duration occurred at 18:00 to 20:00, was given by 16L:8D and 8L:4D:4L:8D (>20.5 min/hen) (P < 0.05). The highest sleeping frequency was given by the 12L:2D:4L:6D, the lowest was given by the 12L:12D (P < 0.05). At 6:00 to 8:00, the shortest sleeping duration was given by 12L:2D:4L:6D (1.5 s/hen), at 18:00 to 20:00, the longest given by 8L:4D:4L:8D (14.3 s/hen) (P < 0.05). Lighting pattern and photoperiod alone or in interaction had no effect on egg-laying rate during 22 to 30 wk (P > 0.05), but had significant effects on ovarian weight, oviduct weight, oviduct length, the number of LYF and SYF (P < 0.05), and the continuous groups were all significantly higher than the intermittent groups (P < 0.05). The present study indicated that light regimes affected the frequency and duration of circadian behavior in BYC laying hens, and the continuous light was more beneficial to the reproductive development than the intermittent light in the early laying period.

Keywords: circadian behavior; lighting pattern; photoperiod; reproductive parameters.

Figure 1

Effects of light regime on circadian change of feeding frequency of BYC. Note: 16L:8D, 6:00–22:00; 12L:2D:4L:6D, 6:00–18:00. 20:00–24:00; 14L:10D, 6:00–20:00; 10L:2D:4L:8D, 6:00–16:00,18:00–22:00; 12L:12D, 6:00–18:00; 8L:4D:4L:8D, 6:00–14:00,18:00–22:00. Abbreviation: BYC, Beijing You Chicken.

Figure 2

Effects of light regime on average feeding duration of BYC. Abbreviation: BYC, Beijing You Chicken.

Figure 3

Effects of light regime on circadian change of egg-laying frequency of BYC. Abbreviation: BYC, Beijing You Chicken.

Figure 4

Effects of light regime on average egg-laying duration of BYC. Abbreviation: BYC, Beijing You Chicken.

Figure 5

Effects of light regime on circadian change of sleeping frequency of BYC. Abbreviation: BYC, Beijing You Chicken.

Figure 6

Effects of light regime on average sleeping duration of BYC. Abbreviation: BYC, Beijing You Chicken.