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. 2022 Feb 20;12(4):e4332.
doi: 10.21769/BioProtoc.4332.

Measuring Endogenous GA and IAA

Yunping Chen  1 , Zhiwu Dan  1 , Shaoqing Li  1
Affiliations

Measuring Endogenous GA and IAA

Yunping Chen et al. Bio Protoc. .

Abstract

Plant hormones regulate many physiological processes that largely influence growth, differentiation, and development. Contents of phytohormones were analyzed using a liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) system. This protocol describes a detailed procedure to extract and quantify indole-3-acetic acid (IAA) and gibberellin acid (GA) in rice (Oryza sativa) tissues using high-performance liquid chromatography (HPLC)-based method.

Keywords: GA; HPLC; IAA; Oryza sativa; Phytohormones.

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Conflict of interest statement

Competing interestsThe authors declare no financial or non-financial competing interests.

References

    1. Chen Y., Dan Z., Gao F., Chen P., Fan F. and Li S.(2020). Rice GROWTH-REGULATING FACTOR7 Modulates Plant Architecture through Regulating GA and Indole-3-Acetic Acid Metabolism. Plant Physiol 184(1): 393-406. - PMC - PubMed
    1. Chen Y., Dan Z. and Li S.(2020). Rice GROWTH-REGULATING FACTOR 7 controls tiller number by regulating strigolactone synthesis. Plant Signal Behav 15(11): 1804685. - PMC - PubMed
    1. Luo X., Zheng J., Huang R., Huang Y., Wang H., Jiang L. and Fang X.(2016). Phytohormones signaling and crosstalk regulating leaf angle in rice. Plant Cell Rep 35(12): 2423-2433. - PubMed
    1. Owen S. J. and Abrams S. R.(2009). Measurement of plant hormones by liquid chromatography-mass spectrometry. Methods Mol Biol 495: 39-51. - PubMed
    1. Prado M. J., Largo A., Dominguez C., Gonzalez M. V., Rey M. and Centeno M. L.(2014). Determination of abscisic acid and its glucosyl ester in embryogenic callus cultures of Vitis vinifera in relation to the maturation of somatic embryos using a new liquid chromatography-ELISA analysis method. J Plant Physiol 171(10): 852-859. - PubMed

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