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. 2022 Mar-Apr;12(2):268-272.
doi: 10.1016/j.jobcr.2022.03.006. Epub 2022 Mar 16.

Effect of nonsurgical periodontal therapy on gingival crevicular fluid levels of Interleukin-35 in patients with periodontitis

Arshiya Goswamy  1 , Mayank Hans  2 , Veenu Madaan Hans  2 , Vidushi Sheokand  3 , Harpreet Singh Grover  4
Affiliations

Effect of nonsurgical periodontal therapy on gingival crevicular fluid levels of Interleukin-35 in patients with periodontitis

Arshiya Goswamy et al. J Oral Biol Craniofac Res. 2022 Mar-Apr.

Abstract

Objective: Interleukin (IL)-35 is a comparatively novel immunosuppressive cytokine produced by T-regulatory cells, the purpose of which in periodontal well being and disease still eludes the researchers. This study intends to measure and compare the levels of IL-35 in gingival crevicular fluid (GCF) taken from periodontitis patients before and at first, second and third week post non-surgical periodontal therapy.

Methodology: ology: Twenty patients having generalized chronic periodontitis (mean age of 36.25 ± 5.12 years) with moderate to severe disease were assessed clinically for the following parameters: plaque index, gingival index, probing pocket depth, and clinical attachment loss. GCF samples were collected from deepest pockets before performing a full mouth non-surgical periodontal therapy. GCF samples were again collected at 1st, 2nd, and 3rd week after non-surgical periodontal therapy and, IL-35 levels in the GCF samples were measured using an ELISA kit.

Results: All the clinical parameters improved significantly over time from baseline to 3rd week. The results for plaque index, gingival index, and probing pocket depth were highly significant (p < 0.001) and significant (p < 0.05) for clinical attachment loss. The IL-35 concentration in GCF increased post periodontal therapy from baseline till third week and results were statistically highly significant (p < 0.001). A significant negative correlation (p < 0.001) was found between clinical parameters and IL-35 levels.

Conclusions: With the healing of the previously diseased periodontal tissues, the levels of IL-35 in GCF increases significantly. Therefore, IL-35 can be considered as potential inflammatory marker of periodontal health and disease.

Keywords: Gingival crevicular fluid; Interleukin-35; Non-surgical periodontal therapy; Periodontitis.

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Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
Correlation between plaque index and GCF Interleukin-35 levels.
Fig. 2
Fig. 2
Correlation between gingival index and GCF Interleukin-35 levels.
Fig. 3
Fig. 3
Correlation between pocket probing depth and GCF Interleukin-35 levels.
Fig. 4
Fig. 4
Correlation between clinical attachment loss and GCF Interleukin-35 levels.
See this image and copyright information in PMC

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