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. 2022 Jan-Feb;54(1):33-40.
doi: 10.4103/ijp.IJP_557_20.

Studies on biochemical, oxidative and genotoxicity alterations following vas blockage with reversible inhibition of sperm under guidance and reversal in rats

Abdul S Ansari  1 , Mubarik Hussain  2 , Sadi Rehan Khan  1 , Ayesha Badar  1 , Barkha Khilwani  1 , Nirmal Kumar Lohiya  1
Affiliations

Studies on biochemical, oxidative and genotoxicity alterations following vas blockage with reversible inhibition of sperm under guidance and reversal in rats

Abdul S Ansari et al. Indian J Pharmacol. 2022 Jan-Feb.

Abstract

Context: Vas obstruction with reversible inhibition of sperm under guidance (RISUG) for contraception and its reversal, may cause oxidative stress or inimical effects on male reproductive functions.

Objective: To evaluate the biochemical and genotoxicity at the level of reactive oxygen species (ROS) following vas occlusion with RISUG and its reversal by Dimethyl sulphoxide (DMSO) and 5% NaHCO3 in Wistar albino rats.

Settings and design: Animals were divided into seven groups (n = 10), namely sham-operated control, short-term vas occlusion with RISUG for 90 days, reversal with DMSO and 5% NaHCO3, long-term vas occlusion with RISUG for 360 days, reversal with DMSO and 5% NaHCO3.

Materials and methods: Biochemical markers in reproductive tissues, hematology, serum biochemistry, serum electrolytes and ROS measuring indicators, e.g., lipid peroxidation, superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase were examined.

Statistical analysis used: One-way analysis of variance test was performed for analyses of data obtained in this study using the SPSS 10.0 software (SPSS Inc., Chicago, IL, USA).

Results: The tissue and clinical chemistry did not show appreciable alterations in RISUG injected and reversal Groups (II-VII) as compared to sham control. The genotoxicity and various ROS markers fluctuated within control limits following short- and long-term vas occlusion and reversal.

Conclusions: The study suggested that the reversal procedures, following RISUG contraception, were not associated with any kind of toxicological manifestations.

Keywords: Oxidative stress; rats; reversal; reversible inhibition of sperm under guidance.

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Conflict of interest statement

None

Figures

Figure 1
Figure 1
(a-d) Biochemical markers in testes of sham control, vas occluded and reversed male albino rats
Figure 2
Figure 2
(a-d) Biochemical markers in epididymis of sham control, vas occluded and reversed male albino rats
Figure 3
Figure 3
(a-e) Reactive oxygen species and antioxidant system in testes of sham control, vas occluded and reversed male albino rats
Figure 4
Figure 4
(a-e) Reactive oxygen species and antioxidant system in epididymis of sham control, vas occluded and reversed male albino rats
See this image and copyright information in PMC

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References

    1. Kennedy AR, Troll W, Little JB. Role of free radicals in the initiation and promotion of radiation transformation in vitro. Carcinogenesis. 1984;5:1213–8. - PubMed
    1. Agarwal A, Virk G, Ong C, du Plessis SS. Effect of oxidative stress on male reproduction. World J Mens Health. 2014;32:1–17. - PMC - PubMed
    1. Khilwani B, Badar A, Ansari AS, Lohiya NK. RISUG® as a male contraceptive: Journey from bench to bedside. Basic Clin Androl. 2020;30:2. - PMC - PubMed
    1. Lohiya NK, Alam I, Hussain M, Khan SR, Ansari AS. RISUG: An intravasal injectable male contraceptive. Indian J Med Res. 2014;140(Suppl):S63–72. - PMC - PubMed
    1. Ansari AS, Badar A, Balasubramanian K, Lohiya NK. Contraception with RISUG® and functional reversal through DMSO and NaHCO3 in male rabbits. Asian J Androl. 2017;19:389–95. - PMC - PubMed