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. 2022 Mar 28;19(1):45-50.
doi: 10.4274/tjod.galenos.2022.44845.

Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study

Nur Dokuzeylül Güngör  1 , Kağan Güngör  2 , Arzu Yurci  3 , Kaan Cil  4 , Şafak Hatırnaz  5
Affiliations

Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study

Nur Dokuzeylül Güngör et al. Turk J Obstet Gynecol. .

Abstract

Objective: To investigate the impact of laparoscopic ovarian drilling (LOD) on the expression of endometrial NFκB p65 (Rel A) in women with clomiphene-resistant polycystic ovary syndrome (PCOS).

Materials and methods: The study group comprised 25 normal-weight women with PCOS undergoing LOD and 14 control women without PCOS. Endometrial NF-κB p65 levels evaluated before and after LOD following immunohistochemical staining. The semiquantitative method was used to evaluate the intensity of NF-κB p65 levels. NF-κB p65 was found to higher in the endometrium of patients with PCOS compared to controls. LOD leads to significant down-regulation in endometrial NF-κB p65 expression. NF-κB p65 expression of PCOS and fertile control were similar after LOD. After LOD, H-score values decreased approximately 3-fold. The H-score of the control subjects was lower than the preoperative and postoperative H-score values of the control women with ovarian cyst.

Results: Expression of endometrial NF-κB p65 did not change following ovarian cystectomy. The laterality of the ovarian cyst did not cause any change in preoperative H-score values.

Conclusion: By downregulating the endometrial NF-κB p65 expression LOD improved physiological inflammation in women with PCOS.

Keywords: PCOS; clomiphene; endometrial inflammation; nuclear factor κB; ovarian drilling.

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Conflict of interest statement

Conflict of Interest: No conflict of interest was declared by the authors.

Figures

Figure 1
Figure 1
High endometrial NF-κB p65 immunoreactivity in women with PCOS localized in luminal and glandular epithelium before LOD (A, red arrowheads, X20). Significantly decreased NF-κB p65 expression after LOD (B, arrow, X20). Normal endometrial NF-κB p65 expression in ovarian cyst group before cystectomy (C, arrowheads X20). Insignificant decline in NF-κB p65 expression following cystectomy (D, arrowhead X10) PCOS: Polycystic ovarian syndrome, LOD: Laparoscopic ovarian drilling
See this image and copyright information in PMC

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