Metagenome-Assembled Genomes from a Microbiome Converting Xylose to Medium-Chain Carboxylic Acids

Abstract

There is growing interest in producing beneficial products from wastes using microbiomes. We previously performed multiomic analyses of a bioreactor microbiome that converted carbohydrate-rich lignocellulosic residues to medium-chain carboxylic acids. Here, we present draft metagenome-assembled genomes from this microbiome, obtained from reactors in which xylose was the primary carbon source.

FIG 1

Phylogenetic tree constructed from recovered MAGs and related genomes. For each recovered MAG, three to five of the most closely related genomes were selected based on GTDB-Tk results (e.g., genus, species, average nucleotide identity [ANI], and alignment fraction [AF]). Genomes retrieved from NCBI were processed using GTDB-Tk to create concatenated sequences of 120 bacterial single-copy marker genes. The tree was constructed from the sequences using RAxML-NG (13) with 1,000 bootstraps. Filled circles indicate bootstrap values of 100, and all others are labeled as numbers. ACET, Acetobacterales; COR, Coriobacteriales; LAC, Lactobacillales; LCO2, Lachnospirales.