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. 2022:2463:67-80.
doi: 10.1007/978-1-0716-2160-8_6.

Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10× Genomics' Single-Cell RNA-Seq

Dominic Paul Lee  1 , Wang Jiehao Ray  1 , Tan Pee Mei  2 , Shawn Hoon  2 , Jonathan Scolnick  1 , Gene W Yeo  3   4
Affiliations

Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10× Genomics' Single-Cell RNA-Seq

Dominic Paul Lee et al. Methods Mol Biol. 2022.

Abstract

Recent advances in multimodal approaches toward single-cell analyses present valuable data points that can complement standard flow cytometry data. In particular, the overlay of cell-surface proteome data with gene expression analysis presents a necessary advancement, particularly in the field of immunology. Here we describe a copper-free click chemistry method for the generation of antibody-oligonucleotide complexes and present the steps for its employment in the context of the 10× genomics droplet-based single-cell RNA-seq workflow, providing a method for coupling proteomic and transcriptomic analyses in an efficient and cost-effect manner.

Keywords: 10× Genomics; Antibody conjugation; Multi-omics; SPAAC; Single-cell RNA-seq; Single-cell proteomics.

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