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. 2022:2463:67-80.
doi: 10.1007/978-1-0716-2160-8_6.

Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10× Genomics' Single-Cell RNA-Seq

Dominic Paul Lee  1 , Wang Jiehao Ray  1 , Tan Pee Mei  2 , Shawn Hoon  2 , Jonathan Scolnick  1 , Gene W Yeo  3   4
Affiliations

Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10× Genomics' Single-Cell RNA-Seq

Dominic Paul Lee et al. Methods Mol Biol. 2022.

Abstract

Recent advances in multimodal approaches toward single-cell analyses present valuable data points that can complement standard flow cytometry data. In particular, the overlay of cell-surface proteome data with gene expression analysis presents a necessary advancement, particularly in the field of immunology. Here we describe a copper-free click chemistry method for the generation of antibody-oligonucleotide complexes and present the steps for its employment in the context of the 10× genomics droplet-based single-cell RNA-seq workflow, providing a method for coupling proteomic and transcriptomic analyses in an efficient and cost-effect manner.

Keywords: 10× Genomics; Antibody conjugation; Multi-omics; SPAAC; Single-cell RNA-seq; Single-cell proteomics.

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References

    1. Lee J, Hyeon DY, Hwang D (2020) Single-cell multiomics: technologies and data analysis methods. Exp Mol Med 52:1428–1442. https://doi.org/10.1038/s12276-020-0420-2 - DOI - PubMed - PMC
    1. Peterson VM, Zhang KX, Kumar N et al (2017) Multiplexed quantification of proteins and transcripts in single cells. Nat Biotechnol 35:936–939. https://doi.org/10.1038/nbt.3973 - DOI - PubMed
    1. Stoeckius M, Hafemeister C, Stephenson W et al (2017) Simultaneous epitope and transcriptome measurement in single cells. Nat Methods 14:865–868. https://doi.org/10.1038/nmeth.4380 - DOI - PubMed - PMC
    1. Hershey JWB, Sonenberg N, Mathews MB (2012) Principles of translational control: an overview. Cold Spring Harb Perspect Biol 4:a011528–a011528. https://doi.org/10.1101/cshperspect.a011528 - DOI - PubMed - PMC
    1. Truitt ML, Ruggero D (2016) New frontiers in translational control of the cancer genome. Nat Rev Cancer 16:288–304. https://doi.org/10.1038/nrc.2016.27 - DOI - PubMed - PMC

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