Tracking of NK Cells by Positron Emission Tomography Using 89Zr-Oxine Ex Vivo Cell Labeling

doi:10.1007/978-1-0716-2160-8_11

. 2022:2463:153-161.

doi: 10.1007/978-1-0716-2160-8_11.

Tracking of NK Cells by Positron Emission Tomography Using ⁸⁹Zr-Oxine Ex Vivo Cell Labeling

Noriko Sato¹, Lawrence P Szajek², Peter L Choyke³

Affiliations

¹ Molecular Imaging Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. saton@mail.nih.gov.
² PET Department, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
³ Molecular Imaging Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

PMID: 35344173
DOI: 10.1007/978-1-0716-2160-8_11

Tracking of NK Cells by Positron Emission Tomography Using ⁸⁹Zr-Oxine Ex Vivo Cell Labeling

Noriko Sato et al. Methods Mol Biol. 2022.

. 2022:2463:153-161.

doi: 10.1007/978-1-0716-2160-8_11.

Authors

Noriko Sato¹, Lawrence P Szajek², Peter L Choyke³

Affiliations

¹ Molecular Imaging Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. saton@mail.nih.gov.
² PET Department, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
³ Molecular Imaging Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

PMID: 35344173
DOI: 10.1007/978-1-0716-2160-8_11

Abstract

A ⁸⁹Zr-oxine ex vivo cell labeling method for tracking various cells by positron emission tomography (PET) imaging has recently been developed. ⁸⁹Zr-oxine is synthesized from oxine and ⁸⁹Zr-chloride, which was converted from ⁸⁹Zr-oxalate, with neutralization. To track migration of natural killer (NK) cells in vivo in real time by PET imaging, NK cells are labeled with ⁸⁹Zr-oxine ex vivo and infused to a recipient. The labeling is performed by mixing ⁸⁹Zr-oxine solution to NK cell suspension at room temperature, followed by washing. Care should be taken to label the cells at optimal radioactivity doses that maintain their viability and functionality. ⁸⁹Zr-oxine labeled NK cells can be tracked for their migration and distribution by PET/computed tomography imaging for at least 7 days. Of note, this protocol is applicable to other types of cells.

Keywords: 89Zr-oxine; Cell tracking; Natural killer cells; Positron emission tomography; Zirconium-89.

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References

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[1] Ruggeri L, Aversa F, Martelli MF, Velardi A (2006) Allogeneic hematopoietic transplantation and natural killer cell recognition of missing self. Immunol Rev 214:202–218 - DOI

[2] Ruggeri L, Aversa F, Martelli MF, Velardi A (2006) Allogeneic hematopoietic transplantation and natural killer cell recognition of missing self. Immunol Rev 214:202–218 - DOI

[3] Caligiuri MA (2008) Human natural killer cells. Blood 112:461–469 - DOI

[4] Caligiuri MA (2008) Human natural killer cells. Blood 112:461–469 - DOI

[5] Vivier E, Raulet DH, Moretta A, Caligiuri MA, Zitvogel L, Lanier LL, Yokoyama WM, Ugolini S (2011) Innate or adaptive immunity? The example of natural killer cells. Science 331:44–49 - DOI

[6] Vivier E, Raulet DH, Moretta A, Caligiuri MA, Zitvogel L, Lanier LL, Yokoyama WM, Ugolini S (2011) Innate or adaptive immunity? The example of natural killer cells. Science 331:44–49 - DOI

[7] Bern MD, Parikh BA, Yang L, Beckman DL, Poursine-Laurent J, Yokoyama WM (2019) Inducible down-regulation of MHC class I results in natural killer cell tolerance. J Exp Med 216:99–116 - DOI

[8] Bern MD, Parikh BA, Yang L, Beckman DL, Poursine-Laurent J, Yokoyama WM (2019) Inducible down-regulation of MHC class I results in natural killer cell tolerance. J Exp Med 216:99–116 - DOI

[9] Bjorklund AT, Carlsten M, Sohlberg E, Liu LL, Clancy T, Karimi M, Cooley S, Miller JS, Klimkowska M, Schaffer M, Watz E, Wikstrom K, Blomberg P, Wahlin BE, Palma M, Hansson L, Ljungman P, Hellstrom-Lindberg E, Ljunggren HG, Malmberg KJ (2018) Complete remission with reduction of high-risk clones following haploidentical NK-cell therapy against MDS and AML. Clin Cancer Res 24:1834–1844 - DOI

[10] Bjorklund AT, Carlsten M, Sohlberg E, Liu LL, Clancy T, Karimi M, Cooley S, Miller JS, Klimkowska M, Schaffer M, Watz E, Wikstrom K, Blomberg P, Wahlin BE, Palma M, Hansson L, Ljungman P, Hellstrom-Lindberg E, Ljunggren HG, Malmberg KJ (2018) Complete remission with reduction of high-risk clones following haploidentical NK-cell therapy against MDS and AML. Clin Cancer Res 24:1834–1844 - DOI

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Tracking of NK Cells by Positron Emission Tomography Using ⁸⁹Zr-Oxine Ex Vivo Cell Labeling

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Tracking of NK Cells by Positron Emission Tomography Using ⁸⁹Zr-Oxine Ex Vivo Cell Labeling

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