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. 2022:2463:235-250.
doi: 10.1007/978-1-0716-2160-8_17.

Organoid Co-culture Methods to Capture Cancer Cell-Natural Killer Cell Interactions

Isaac S Chan  1   2 , Andrew J Ewald  3   4
Affiliations

Organoid Co-culture Methods to Capture Cancer Cell-Natural Killer Cell Interactions

Isaac S Chan et al. Methods Mol Biol. 2022.

Abstract

Metastasis is a complex process that has been historically difficult to model in culture. Host immune responses play critical roles in restraining and promoting metastatic tumor cells. Here we describe a method of 3D organotypic co-culture of natural killer cells and tumor organoids to capture interactions between the two cellular populations. These assays can be used to model key aspects of metastatic biology and to screen for the effectiveness of agents that stimulate natural killer cell cytotoxicity.

Keywords: 3D culture; Antibody-dependent cell-mediated cytotoxicity; Breast cancer; Cancer metastasis; Immune escape; Innate immune system; Natural killer cells; Organoids; Organotypic co-culture; Tumor immunosurveillance.

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References

    1. Lambert AW, Pattabiraman DR, Weinberg RA (2017) Emerging biological principles of metastasis. Cell 168(4):670–691 - DOI
    1. Esposito M, Ganesan S, Kang Y (2021) Emerging strategies for treating metastasis. Nat Cancer 2(3):258–270 - DOI
    1. Roarty K, Echeverria GV (2021) Laboratory models for investigating breast cancer therapy resistance and metastasis. Front Oncol 11:645698 - DOI
    1. Cheung KJ, Gabrielson E, Werb Z, Ewald AJ (2013) Collective invasion in breast cancer requires a conserved basal epithelial program. Cell 155(7):1639–1651 - DOI
    1. Cheung KJ, Padmanaban V, Silvestri V, Schipper K, Cohen JD, Fairchild AN et al (2016) Polyclonal breast cancer metastases arise from collective dissemination of keratin 14-expressing tumor cell clusters. Proc Natl Acad Sci U S A 113(7):E854–E863 - DOI

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