Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 May 18;23(10):e202200066.
doi: 10.1002/cbic.202200066. Epub 2022 Apr 7.

Photocontrollable Probes for Mitochondrial Protein Profiling

Shuhong Guo  1 , Chaonan Yuan  1 , Wenjie Lang  1 , Danqi Hong  1 , Jian Liu  1 , Jintao Huang  1 , Jia Dong  1 , Jingyan Ge  1
Affiliations

Photocontrollable Probes for Mitochondrial Protein Profiling

Shuhong Guo et al. Chembiochem. .

Abstract

The mitochondrion is the core site of cell signaling, energy metabolism and biosynthesis. Here, taking advantage of activity-based probes, we synthesized two photocontrollable probes (YGH-1 and YGH-2), composed of a mitochondrial localization moiety "triphenylphosphonium", a photo-triggered group to achieve spatially and temporally controlled protein capture, and an alkyne group to enrich the labeled protein. Proteomic validation was further carried out to facilitate identification of the mitochondrial proteome in HeLa cells. The results show that half of the identified protein hits (∼300) labeled by YGH-1 and YGH-2 belong to mitochondria, and are mostly localized in the mitochondrial matrix and inner mitochondrial membrane. Our results provide a new tool for spatial and temporal analysis of subcellular proteomes.

Keywords: mitochondrial proteome; photo-crosslinker; photocontrol; quinone methide.

PubMed Disclaimer

References

    1. J. R. Friedman, J. Nunnari, Nature 2014, 505, 335-343.
    1. X. Guo, N. Yang, W. Ji, H. Zhang, X. Dong, Z. Zhou, L. Li, H.-M. Shen, S. Q. Yao, W. Huang, Adv. Mater. 2021, 33, e2007778.
    1. C. Frezza, S. Cipolat, L. Scorrano, Nat. Protoc. 2007, 2, 287-295.
    1. P. J. Thul, L. Åkesson, M. Wiking, D. Mahdessian, A. Geladaki, H. Ait Blal, T. Alm, A. Asplund, L. Björk, L. M. Breckels, A. Bäckström, F. Danielsson, L. Fagerberg, J. Fall, L. Gatto, C. Gnann, S. Hober, M. Hjelmare, F. Johansson, S. Lee, C. Lindskog, J. Mulder, C. M. Mulvey, P. Nilsson, P. Oksvold, J. Rockberg, R. Schutten, J. M. Schwenk, Å. Sivertsson, E. Sjöstedt, M. Skogs, C. Stadler, D. P. Sullivan, H. Tegel, C. Winsnes, C. Zhang, M. Zwahlen, A. Mardinoglu, F. Pontén, K. von Feilitzen, K. S. Lilley, M. Uhlén, E. Lundberg, Science 2017, 365, eaal3321.
    1. H. Zhu, T. Tamura, I. Hamachi, Curr. Opin. Chem. Biol. 2019, 48, 1-7.

Publication types

LinkOut - more resources