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. 2022 Mar 19:2022:6571272.
doi: 10.1155/2022/6571272. eCollection 2022.

Expression and Prognosis Value of the KLF Family Members in Colorectal Cancer

Zhongting Huang  1 , Haibin He  1 , Feng Qiu  1 , Hailong Qian  1
Affiliations

Expression and Prognosis Value of the KLF Family Members in Colorectal Cancer

Zhongting Huang et al. J Oncol. .

Abstract

Krüppel-like factors (KLFs) are some kind of transcriptional regulator that regulates a broad range of cellular functions and has been linked to the development of certain malignancies. KLF expression patterns and prognostic values in colorectal cancer (CRC) have, however, been investigated rarely. To investigate the differential expression, predictive value, and gene mutations of KLFs in CRC patients, we used various online analytic tools, including ONCOMINE, TCGA, cBioPortal, and the TIMER database. KLF2-6, KLF8-10, KLF12-15, and KLF17 mRNA expression levels were dramatically downregulated in CRC tissues, but KLF1, KLF7, and KLF16 mRNA expression levels were significantly elevated in CRC tissues. According to the findings of Cox regression analysis, upregulation of KLF3, KLF5, and KLF6 and downregulation of KLF15 were linked with a better prognosis in CRC. For functional enrichment, our findings revealed that KLF members are involved in a variety of cancer-related biological processes. In colon cancer and rectal cancer, KLFs were also shown to be associated with a variety of immune cells. The findings of this research reveal that KLF family members' mRNA expression levels are possible prognostic indicators for patients with CRC.

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Conflict of interest statement

The authors declare that they have no potential conflicts of interest.

Figures

Figure 1
Figure 1
Transcriptional expression of KLF genes in CRC (ONCOMINE database).
Figure 2
Figure 2
Associations between KLF family genes in CRC.
Figure 3
Figure 3
Expression profile of KLF members in CRC. (a) Heatmap of TCGA samples. (b) Histogram of TCGA sample.
Figure 4
Figure 4
Genetic alterations and association between DNA methylation and expression of KLF genes in CRC. (a) Summary of alterations in differently expressed KLFs in CRC. (b) OncoPrint visual summary of alterations with a query of KLF family members. (c) Association between DNA methylation and expression of KLF genes in CRC.
Figure 5
Figure 5
Kaplan–Meier curves of overall survival in patients with CRC with high and low KLF family mRNA expression.
Figure 6
Figure 6
Multivariate Cox proportional hazard regression analysis of KLF members (KLF3 (a), KLF5 (b), KLF6 (c), and KLF15 (d)) and clinicopathologic features for overall survival in CRC.
Figure 7
Figure 7
The correlation between KLF members (KLF3, KLF5, KLF6, and KLF15) and the abundance of each type of immune cells (B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages, and dendritic cells) using TIMER database.
Figure 8
Figure 8
Gene set enrichment analysis (GSEA) of KLF3 (a), KLF5 (b), KLF6 (c), and KLF15 (d) in patients with CRC using TCGA dataset.
Figure 9
Figure 9
Differences in immune checkpoints between different groups of high and low expressing KLFs.
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