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Review
. 2022 Aug;22(15-16):e2200026.
doi: 10.1002/pmic.202200026. Epub 2022 Apr 7.

Human peripheral blood mononuclear cells: A review of recent proteomic applications

Michal Alexovič  1 , Joshua Raoul Lindner  2 , Peter Bober  1 , Rémi Longuespée  2 , Ján Sabo  1 , Katarina Davalieva  3
Affiliations
Review

Human peripheral blood mononuclear cells: A review of recent proteomic applications

Michal Alexovič et al. Proteomics. 2022 Aug.

Abstract

Human peripheral blood mononuclear cells (PBMCs) represent a sentinel blood sample which reacts to different pathophysiological stimuli in the form of immunological responses/immunophenotypic changes. The study of molecular content of PBMCs can provide better understanding of immune processes giving the possibility of monitoring the health conditions of the host organism. Proteomic analysis of PBMCs can achieve mentioned goal as important immune-related biomarkers are easily accessible for analysis. PBMCs have been gaining attention in different research areas including preclinical or clinical investigations. In this review, recent applications of proteomic analysis of PBMCs are described and discussed. Approaches are divided based on different proteomic workflows such as in-gel, in-solution and on-filter modes. The effect of various diseases such as autoimmune, cancer, neurodegenerative, viral, metabolic, and various immune stimulations such as radiation, vaccine, corticosteroids over PBMCs proteome, are described with emphasis on promising protein biomarker candidates.

Keywords: human peripheral blood mononuclear cells (PBMCs); mass spectrometry; protein biomarkers; proteomics; sample preparation.

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References

REFERENCES

    1. Belczacka, I., Latosinska, A., Metzger, J., Marx, D., Vlahou, A., Mischak, H., & Frantzi, M. (2019). Proteomics biomarkers for solid tumors: Current status and future prospects. Mass Spectrometry Reviews, 38(1), 49-78. https://doi.org/10.1002/mas.21572
    1. Zhang, B., Whiteaker, J. R., Hoofnagle, A. N., Baird, G. S., Rodland, K. D., & Paulovich, A. G. (2019). Clinical potential of mass spectrometry-based proteogenomics. Nature Reviews Clinical oncology, 16(4), 256-268. https://doi.org/10.1038/s41571-018-0135-7
    1. Yokota, H. (2019). Applications of proteomics in pharmaceutical research and development. Biochimica et Biophysica Acta - Proteins and Proteomics, 1867(1), 17-21. https://doi.org/10.1016/j.bbapap.2018.05.008
    1. Bober, P., Alexovič, M., Tomková, Z., Kilík, R., & Sabo, J. (2019). RHOA and mDia1 promotes apoptosis of breast cancer cells via a high dose of doxorubicin treatment. Open Life Science, 14, 619-627. https://doi.org/10.1515/biol-2019-0070
    1. Fu, Q., Murray, C. I., Karpov, O. A., & Van Eyk, J. E. (2021). Automated proteomic sample preparation: The key component for high throughput and quantitative mass spectrometry analysis. Mass Spectrometry Reviews, e21750. https://doi.org/10.1002/mas.21750

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