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. 2022 Aug;45(4):1780-1799.
doi: 10.1007/s10753-022-01660-x. Epub 2022 Mar 29.

The Integrated Analysis of Transcriptomics and Metabolomics Unveils the Therapeutical Effect of Asiatic Acid on Alcoholic Hepatitis in Rats

Siyun Chen  1 , Yushen Huang  1 , Hongmei Su  1 , Wuchang Zhu  1 , Yuanyuan Wei  1 , Yan Long  1 , Yanxia Shi  1 , Jinbin Wei  2   3
Affiliations

The Integrated Analysis of Transcriptomics and Metabolomics Unveils the Therapeutical Effect of Asiatic Acid on Alcoholic Hepatitis in Rats

Siyun Chen et al. Inflammation. 2022 Aug.

Abstract

The present study was to investigate the therapeutical effects and mechanisms of Asiatic acid from Potentilla chinensis against alcoholic hepatitis. Rats were intragastrically fed with alcohol for 12 weeks to induce alcoholic hepatitis and then treated with various drugs for further 12 weeks. The results showed that Asiatic acid significantly alleviated liver injury caused by alcohol in rats, as evidenced by the improved histological changes and the lower levels of AST, ALT, and TBIL. Besides, Asiatic acid significantly enhanced the activity of ADH and ALDH, promoting alcohol metabolism. Asiatic acid suppressed CYP2E1 activity and NADP+/NADPH ratio, resulting in low ROS production. Further study revealed that Asiatic acid markedly reduced hepatocyte apoptosis by regulating the expression levels of apoptosis-related protein. Moreover, Asiatic acid could regulate the Nrf2 and NF-κB signaling pathway, attenuating oxidative stress and inflammation as a result. Interestingly, the comprehensive analysis of transcriptomics and metabolomics indicated that Asiatic acid inhibited the gene expression of Gpat3 and thereby affected the biosynthesis of the metabolites (1-acyl-Sn-glycerol-3-phosphocholine, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine), regulating the glycerophospholipid metabolism pathway and ultimately ameliorating hepatocyte damage. In conclusion, this study demonstrates that Asiatic acid can ameliorate alcoholic hepatitis by modulating the NF-κB and Nrf2 signaling pathways and the glycerophospholipid metabolism pathway, which may be developed as a potential medicine for the treatment of alcoholic hepatitis.

Keywords: Alcoholic hepatitis; Asiatic acid; Glycerophospholipid metabolism pathway; NF-κB pathway; Nrf2 pathway.

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References

    1. Wan, Y., H. Wu, Y. Li, Z. Xu, J. Yang, C. Liu, Y. He, M. Wang, X. Wu, and Y. Zhang. 2020. viaTSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis Suppression of STAT3 Activation. Frontiers in pharmacology 11: 10. - DOI
    1. Xiang, X., S. Hwang, D. Feng, V. Shah, and B. Gao. 2020. Interleukin-22 in alcoholic hepatitis and beyond. Hepatology international 14: 667–676. - DOI
    1. Takeuchi, M., P. Vidigal, M. Guerra, M. Hundt, M. Robert, M. Olave-Martinez, S. Aoki, T. Khamphaya, R. Kersten, E. Kruglov, et al. 2021. Neutrophils interact with cholangiocytes to cause cholestatic changes in alcoholic hepatitis. Gut 70: 342–356. - PubMed
    1. Gala, K., and V. Vatsalya. 2020. Emerging noninvasive biomarkers, and medical management strategies for alcoholic hepatitis: present understanding and scope. Cells 9.
    1. Waleed, M., M. Abdallah, Y. Kuo, J. Arab, R. Wong, and A. Singal. 2020. Higher Frequency of Hospital-Acquired Infections but Similar In-Hospital Mortality Among Admissions With Alcoholic Hepatitis at Academic vs. Non-academic Centers. Frontiers in physiology 11: 594138.

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