Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Sep;53(3):1645-1655.
doi: 10.1007/s42770-022-00736-y. Epub 2022 Mar 29.

Fractionation of hexane extracts from Achyrocline satureioides and their biological activities against Paenibacillus larvae

Natalia Tonello  1 , Diana Pimentel Betancurt  1 , Carlos Leonel Huallpa  2 , Juan Miguel Marioli  1 , Marcela Beatriz Moressi  1 , María de Las Mercedes Oliva  2 , Fabiana D'Eramo  3
Affiliations

Fractionation of hexane extracts from Achyrocline satureioides and their biological activities against Paenibacillus larvae

Natalia Tonello et al. Braz J Microbiol. 2022 Sep.

Abstract

Previous studies carried out in our laboratory described the antimicrobial activity of the whole hexanic extract (HE) of Achyrocline satureioides (Lam.) DC against Paenibacillus larvae, the causal agent of American Foulbrood (AFB) a disease of the honey bee larvae. In this study, the HE was partitioned into five main fractions by chromatographic techniques leading to the isolation of four known compounds: two prenylated phloroglucinol α-pyrones (1 and 3), 5,7-dihydroxy-3,8-dimethoxyflavone (gnaphaliin A) (2), and 23-methyl-6-O-demethylauricepyrone (4). Isolated compounds were further analyzed towards structural elucidation using 1H RMN and 13C RMN spectroscopic techniques. For the first time, the antimicrobial activity of the isolated compounds was evaluated against P. larvae strains by broth microdilution method and compared with that of the whole HE. Compounds 1-4 displayed minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values ranging between 0.07 and 62.5 μg/mL and 0.26 and 12.5 μg/mL, respectively. The lowest MIC and MBC values were obtained with compounds 3 and 4, respectively. The antimicrobial activity of each single compound and the combination of them showed that the presence of all compounds is needed for the antimicrobial efficacy of whole HE.

Keywords: Achyrocline satureioides; American foulbrood; Bees; Honey; Paenibacillus larvae.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Molecular structure of compounds 1 to 4
Fig. 2
Fig. 2
MIC isobolograms for combinations of compounds 1 and 3 against Pl 9 and Pl T.
See this image and copyright information in PMC

References

    1. Genersh E. American foulbrood in honeybees and its causative agent. Paenibacillus larvae J Invertebr Pathol. 2010;103:S10–S19. doi: 10.1016/j.jip.2009.06.015. - DOI - PubMed
    1. Lindström A, Fries I. Sampling of adult bees for detection of American foulbrood (Paenibacillus larvae subsp. larvae) spores in honey bee (Apis mellifera) colonies. J Apic Res. 2015;44(2):82–86. doi: 10.1080/00218839.2005.11101154. - DOI
    1. Se recuerda que no está permitido el uso de antibióticos en las colmenas. Available from: www.senasa.gob.ar. Accessed July 3, 2021.
    1. Evans JD. Diverse origins of tetracycline resistance in the honey bee bacterial pathogen. Paenibacillus larvae J Invertebr Pathol. 2003;83:46–50. doi: 10.1016/S0022-2011(03)00039-9. - DOI - PubMed
    1. Alippi AM, López AC, Reynaldi FJ, Grasso DH, Aguilar OM. Evidence for plasmidmediated tetracycline resistance in Paenibacillus larvae, the causal agent of American Foulbrood (AFB) disease in honeybees. Vet Microbiol. 2007;125:290–303. doi: 10.1016/j.vetmic.2007.05.018. - DOI - PubMed

LinkOut - more resources