Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2022 Jun;32(6):505-506.
doi: 10.1038/s41422-022-00649-3.

Amplifying natural antitumor immunity for personalized immunotherapy

Laura K Donovan  1 , Michael D Taylor  2
Affiliations
Comment

Amplifying natural antitumor immunity for personalized immunotherapy

Laura K Donovan et al. Cell Res. 2022 Jun.
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1. The development of personalized Tas TCR-T cells.
(1) Surgical tumor, peri-tumor, normal tissue and blood samples are collected from a patient. (2) Next-generation sequencing is performed on the tissues to identify potent neoantigen epitopes derived from tumor mutations; single-cell RNA-sequencing is performed on the T-cells from tissues and blood samples to identify Tas T-cells. (3) Personalized Tas TCRs are cloned, synthesized and (4) constructed into a lentiviral vector. (5) T-cells isolated from the peripheral blood of patients (leukapheresis) are (6) transduced with these TCRs. (7) Modified Tas TCR T-cells are expanded in vitro to generate sufficient numbers of cells for therapy and (8) re-infused back into the patient.
See this image and copyright information in PMC

Comment on

References

    1. Donovan LK, et al. Nat. Med. 2020;26:720–731. doi: 10.1038/s41591-020-0827-2. - DOI - PMC - PubMed
    1. Northcott PA, et al. Nature. 2017;547:311–317. doi: 10.1038/nature22973. - DOI - PMC - PubMed
    1. Scheper W, et al. Nat. Med. 2019;25:89–94. doi: 10.1038/s41591-018-0266-5. - DOI - PubMed
    1. Tran E, et al. Science. 2014;344:641–645. doi: 10.1126/science.1251102. - DOI - PMC - PubMed
    1. Chandran SS, et al. Lancet Oncol. 2017;18:792–802. doi: 10.1016/S1470-2045(17)30251-6. - DOI - PMC - PubMed

Substances