Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Jun 1;162(1):9-16.
doi: 10.1007/BF00333845.

A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperature

G Kellenberger-Gujer, A J Podhajska, L Caro

A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperature

G Kellenberger-Gujer et al. Mol Gen Genet. .

Abstract

A heat resistant mutant of E. coli dnaAts46 was isolated, which grows normally only at temperatures above 39 degrees. After a temperature shift from 42 degrees to 32 degrees the mutant overproduces DNA relative to protein. This is due to overinitiation of rounds of chromosome replication at low temperature, as indicated by hybridization and other experiments. The mutation is cotransduced by PI with ilv and could not be separated from dnaAts46 by transduction.

PubMed Disclaimer

References

    1. J Mol Biol. 1972 Oct 14;70(3):549-66 - PubMed
    1. J Bacteriol. 1974 Oct;120(1):1-5 - PubMed
    1. J Mol Biol. 1977 Sep 25;115(3):295-314 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1968;33:317-24 - PubMed
    1. J Mol Biol. 1969 Oct 28;45(2):325-36 - PubMed

LinkOut - more resources