Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Apr 21;11(4):e0106521.
doi: 10.1128/mra.01065-21. Epub 2022 Mar 30.

Metagenomes and Metagenome-Assembled Genomes from Substrate-Amended Hot Spring Sediment Incubations from Yellowstone National Park

Affiliations

Metagenomes and Metagenome-Assembled Genomes from Substrate-Amended Hot Spring Sediment Incubations from Yellowstone National Park

Nicholas J Reichart et al. Microbiol Resour Announc. .

Abstract

Here, we report on eight sediment metagenomes obtained from an alkaline hot spring, with their corresponding metagenome-assembled genomes. Samples had been incubated for 48 h with various substrate amendments in conjunction with the amino acid analog l-homopropargylglycine in a study targeted at identifying anabolicly active uncultured thermophilic archaea and bacteria.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIG 1
FIG 1
Sequencing statistics for assembled metagenomes (A and B) and medium- and high-quality MAGs (C to H) generated by the IMG/M pipeline.

References

    1. Eloe-Fadrosh EA, Paez-Espino D, Jarett J, Dunfield PF, Hedlund BP, Dekas AE, Grasby SE, Brady AL, Dong H, Briggs BR, Li WJ, Goudeau D, Malmstrom R, Pati A, Pett-Ridge J, Rubin EM, Woyke T, Kyrpides NC, Ivanova NN. 2016. Global metagenomic survey reveals a new bacterial candidate phylum in geothermal springs. Nat Commun 7:10476. doi:10.1038/ncomms10476. - DOI - PMC - PubMed
    1. Lloyd KG, Steen AD, Ladau J, Yin J, Crosby L. 2018. Phylogenetically novel uncultured microbial cells dominate earth microbiomes. mSystems 3:e00055-18. doi:10.1128/mSystems.00055-18. - DOI - PMC - PubMed
    1. Hatzenpichler R, Scheller S, Tavormina PL, Babin BM, Tirrell DA, Orphan VJ. 2014. In situ visualization of newly synthesized proteins in environmental microbes using amino acid tagging and click chemistry. Environ Microbiol 16:2568–2590. doi:10.1111/1462-2920.12436. - DOI - PMC - PubMed
    1. Hatzenpichler R, Connon SA, Goudeau D, Malmstrom RR, Woyke T, Orphan VJ. 2016. Visualizing in situ translational activity for identifying and sorting slow-growing archaeal-bacterial consortia. Proc Natl Acad Sci USA 113:E4069–E4078. - PMC - PubMed
    1. Hatzenpichler R, Krukenberg V, Spietz RL, Jay ZJ. 2020. Next-generation physiology approaches to study microbiome function at single cell level. Nat Rev Microbiol 18:241–256. doi:10.1038/s41579-020-0323-1. - DOI - PMC - PubMed