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. 2022 Apr;38(4):429-434.
doi: 10.1007/s12264-022-00843-8. Epub 2022 Mar 30.

Parkin Inhibits Static Mechanical Pain by Suppressing Membrane Trafficking of Mechano-transducing Ion Channel TACAN

Yi Lei #  1   2 , Man-Xiu Xie #  3 , Xian-Ying Cao #  4 , Xi Zhang  2 , Yi-Bin Xiao  2 , Xiao-Yu Tian  1 , Yuan-Xin Zhu  1 , Xiao-Long Zhang  5
Affiliations

Parkin Inhibits Static Mechanical Pain by Suppressing Membrane Trafficking of Mechano-transducing Ion Channel TACAN

Yi Lei et al. Neurosci Bull. 2022 Apr.
No abstract available

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Conflict of interest statement

All authors claim that there are no conflicts of interest.

Figures

Fig. 1
Fig. 1
Parkin selectively regulates static mechanical sensitivity. A The mRNA level of Parkin in DRGs of mice on day 5 after CFA treatment (n = 4, two mice mixed as a sample). B The immunoblotting showed the protein level of Parkin in DRG of mice on day 5 after CFA treatment (n = 3, two mice mixed as a sample). C Double immunostaining of Parkin with IB4, CGRP and NF200 in DRG sections of mice. Scale bar, 200 µm. D Behaviors of mice injected with Parkin-siRNA and nontargeting (NT)-siRNA evaluated by the von Frey test (n = 6). E Behaviors of Parkin-overexpressing and control mice evaluated by the von Frey test (n = 5). F–H Effects of overexpression of Parkin on CFA-, SNL- and VCR-induced static mechanical allodynia at different time points (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001. n.s. means not significant. A, B, D, E, independent Student’s t test. F–H, two-way ANOVA followed by Bonferroni’s multiple comparisons test. Error bars indicate SEM.
Fig. 2
Fig. 2
Parkin interacts with TACAN and regulates the membrane trafficking of TACAN in sensory neurons. A, B Co-immunoprecipitation showing the interaction between Parkin and TACAN, or Piezo2 in DRGs. DRG lysate was immunoprecipitated with Parkin antibody and immunoblotted with Parkin, TACAN, and Piezo2 antibody as indicated. This experiment was repeated three times. C, D High-resolution images by structured illumination microscopy showing co-localization between Parkin and TACAN in mouse DRG neurons (C) and human sciatic nerve (D). Scale bars, 5 μm. E–G The membranous (E), cytoplasmic (F), and total (G) TACAN expression in DRG neurons of Parkin-siRNA and nontargeting (NT)-siRNA mice (n = 3, two mice mixed as a sample). H The TACAN membrane trafficking ratio in DRG neurons of Parkin-siRNA and nontargeting (NT)-siRNA mice (n = 3, two mice mixed as a sample). I–K The membranous (I), cytoplasmic (J), and total (K) TACAN expression in DRG neurons of Parkin-overexpressing and control mice (n = 3, two mice mixed as a sample). L The TACAN membrane trafficking ratio in DRG neurons of Parkin-overexpressing and control mice (n = 3, two mice mixed as a sample). Independent Student’s t test. *P < 0.05, **P < 0.01. n.s. means not significant. Error bars indicate SEM.
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