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. 2022 Mar 30;22(1):83.
doi: 10.1186/s12866-022-02499-w.

Effect of phenyllactic acid on silage fermentation and bacterial community of reed canary grass on the Qinghai Tibetan Plateau

Yongxiang Lu  1   2   3 , Ping Li  4   5   6 , Shiqie Bai  7   8 , Shiyong Chen  3 , Man Zhao  2   3 , Wenlong Gou  2 , Minghong You  2 , Qiming Cheng  1
Affiliations

Effect of phenyllactic acid on silage fermentation and bacterial community of reed canary grass on the Qinghai Tibetan Plateau

Yongxiang Lu et al. BMC Microbiol. .

Abstract

Background: This study aimed to investigate the effect of phenyllactic acid as an additive on silage fermentation and bacterial community of reed canary grass (RCG, Phalaris arundinacea L.) on the Qinghai Tibetan Plateau. At the heading stage, RCG was harvested, chopped and ensiled in small bag silos. The silage was treated without (control, 1.0 g/mL sterile water, on a fresh matter basis (FM)) or with phenyllactic acid (PLA, 3 mg/mL, FM), antimicrobial additive (PSB, a mixture of potassium sorbate and sodium benzoate, 2%, FM), lactic acid bacteria inoculant (LABi, L. plantarum + L. curvatus, 1 × 106 cfu/g, FM) and PLA + LABi, and then stored in a dark room at the ambient temperature (5 ~ 15 °C) for 60 days.

Results: Compared with control, PLA decreased lactic acid, acetic acid and ammonia-N contents, and subsequently increased CP content of RCG silage. PLA enhanced the growth of lactic acid bacteria and reduced the count of yeasts (P < 0.05) in RCG silage, with reduced bacterial richness index (Chao1), observed operational taxonomic units and diversity index (Simpson). In relative to control, moreover, PLA and PLA + LABi increased the relative abundance of Lactococcus in RCG silage by 27.73 and 16.93%, respectively.

Conclusions: Therefore, phenyllactic acid at ensiling improved nutritional quality of RCG silage by advancing the disappearance of yeasts and the dominance of Lactococcus.

Keywords: Bacterial population; Bioactive agents; Ensilage; Fermentation quality; Phalaris arundinacea.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Bacterial community composition of reed canary grass silage at genus (a) and specie (b) levels. Silage treated without (CK) or with antimicrobial additive (PSB), phenyllactic acid (PLA), lactic acid inoculant (LABi) and PLA + LABi
Fig. 2
Fig. 2
Fermentation factors affecting bacterial genus level (a) and species level (b) community composition of reed canary grass silage. LA, lactic acid; AA, acetic acid; PA, propionic acid; BA, butyric acid; AN, ammonia-N. * Significant at P < 0.05; ** Significant at P < 0.01
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References

    1. Guo P, Gao P, Li F, Chang S, Wang Z, Yan T. Prediction of metabolizable energy concentrations of herbage on the Qinghai-Tibetan plateau based on Tibetan sheep digestibility data. Animals. 2020;10(3):376. doi: 10.3390/ani10030376. - DOI - PMC - PubMed
    1. Zhou J, Liu H, Zhong C. Apparent digestibility, rumen fermentation, digestive enzymes and urinary purine derivatives in yaks and Qaidam cattle offered forage-concentrate diets with different nitrogen concentration. Livest Sci. 2018;208:14–21. doi: 10.1016/j.livsci.2017.11.020. - DOI
    1. Long RJ, Zhang DG, Wang X, Hu ZZ, Dong SK. Effect of strategic feed supplementation on productive and reproductive performance in yaks. Prev Vete Med. 1999;38(2–3):195–206. doi: 10.1016/S0167-5877(98)00125-1. - DOI - PubMed
    1. Zhong J, Zhijie M, Zhixin C, Hui W, Zhang C, Qiumei J, Jinwei X. Whole genome sequencing of the dzo: genetic implications for high-altitude adaptation, sterility, and milk and meat production. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2018;24(6):835–844. doi: 10.9775/kvfd.2018.20022. - DOI
    1. Wilkinson JM, Rinne M. Highlights of progress in silage conservation and future perspectives. Grass Forage Sci. 2018;73(1):40–52. doi: 10.1111/gfs.12327. - DOI

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