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. 2021 Nov 30;76(5):1289-1295.
doi: 10.22092/ari.2021.356101.1777. eCollection 2021 Nov.

Detection and Sequencing of Iron Superoxide Dismutase Gene in Entamoeba histolytica Isolated from Patients with Diarrhea in Iraq

W F H Al-Zubadi  1 , H K Al-Masoudi  1 , L A Abdul-Lateef  1
Affiliations

Detection and Sequencing of Iron Superoxide Dismutase Gene in Entamoeba histolytica Isolated from Patients with Diarrhea in Iraq

W F H Al-Zubadi et al. Arch Razi Inst. .

Abstract

Parasites are among serious health problems that threaten the public health of humans. Entamoeba histolytica is considered the second or third most common cause of death among parasitic diseases. The present study was conducted to determine the molecular detection and sequencing of iron-containing superoxide dismutase (FeSOD) genes of E. histolytica. This cross-sectional study was carried out within March-August 2020. Genomic DNA from stool samples was extracted using an AccuPrep® stool DNA Extraction Kit. Afterward, four DNA samples were subjected to sequence alignment analysis of the FeSOD gene in local E. histolytica human isolated using a Clustal W alignment tool (Mega 6.0).The iron-containing superoxide dismutase gene was present at 62.8% in 15 out of 24 E. histolytica samples. The results of sequence alignment analysis of the FeSOD gene in local E.histolytica human isolates indicated the number of mutations in each of the 4 isolates. Accordingly, there were 2 (16.66%) silent mutations and 1(6.66%) nonsense mutation. There are a variety of genetic strains of E. histolytica associated with diarrheal illness in Iraqi patients, which are unique to this country.

Keywords: Sequencing; Entamoeba histolytica; FeSOD.

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Figures

Figure 1
Figure 1
Agarose gel electrophoresis image showing the PCR product analysis of diagnostic EHP gene in Entamoeba histolytica from human stool samples Where M: marker (1500-100bp) and Lane (1-24), some positive EHP genes were shown at 135bp PCR product.
Figure 2
Figure 2
Agarose gel electrophoresis image showing the PCR product analysis of FeSOD gene in Entamoeba histolytica from human stool samples Where M: marker (1500-100bp) and Lane (1-20), some positive FeSOD genes were shown at 505bpPCR product.
See this image and copyright information in PMC

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References

    1. Verhagen LM, Incani RN, Franco CR, Ugarte A, Cadenas Y, Ruiz CIS, et al. High Malnutrition Rate in Venezuelan Yanomami Compared to Warao Amerindians and Creoles: Significant Associations WITH Intestinal Parasites and Anemia. PLoS One. 2013;8(10):e77581. - PMC - PubMed
    1. M R-L, RM B-C, EE A. A acetaldehyde alcohol dehycrogenase-2(ELADH2) and clarhrin are involved in internalization of human transferrin by Entamoeba histolytica. Microbiology. 2011;157(1):209 –19. - PubMed
    1. Espinosa A, Perdrizet G, Paz-y-Miño C G, Lanfranchi R, Phay M. Effects of iron depletion on Entamoeba histolytica alcohol dehydrogenase 2 (EhADH2) and trophozoite growth: implications for antiamoebic therapy. J Antimicrob Chemother. 2009;63(4):675–8. - PMC - PubMed
    1. Jeelani G, Husain A, Sato D, Ali V, Suematsu M, Soga T, et al. Two atypical-L-cysteine-regulated NADPH-dependent oxido-reductases involved in redox maintenances L-cysteine and iron reduction, and metronidazole activation in the enteric protozoan Entamoeba histolytica. J Biol Chem. 2010;285(35):26889–99. - PMC - PubMed
    1. Almasoudi H, Sabri M. Study on Cysteine Proteinase Produced by Entamoeba Histolytica. Med j Babylon. 2009

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