doi: 10.1038/274438a0.
Structure and processing of yeast precursor tRNAs containing intervening sequences
- PMID: 353564
- DOI: 10.1038/274438a0
Item in Clipboard
Structure and processing of yeast precursor tRNAs containing intervening sequences
Nature.
.
Abstract
We have isolated a precursor of yeast tRNATyr and shown that it contains an intervening sequence identical to that found in the gene for tRNATyr. The conformation of pre-tRNATyr is similar to that of mature tRNATyr except for the anticodon loop. The loop is sensitive to endonucleolytic cleavage by S1 nuclease near to the ends of the intervening sequence. This pre-tRNA is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding. A crude nuclear extract from yeast contains an excision-ligase activity which will process pre-tRNATyr into mature tRNATyr.
Similar articles
-
Aminoacylation of anticodon loop substituted yeast tyrosine transfer RNA.Biochemistry. 1985 Apr 23;24(9):2354-60. doi: 10.1021/bi00330a034. Biochemistry. 1985. PMID: 3846456
-
Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes.Nature. 1979 Mar 8;278(5700):137-43. doi: 10.1038/278137a0. Nature. 1979. PMID: 368655
-
Yeast tRNA precursor mutated at a splice junction is correctly processed in vivo.Proc Natl Acad Sci U S A. 1981 Jan;78(1):415-9. doi: 10.1073/pnas.78.1.415. Proc Natl Acad Sci U S A. 1981. PMID: 7017715 Free PMC article.
-
Split genes and RNA splicing.Science. 1979 Apr 20;204(4390):264-71. doi: 10.1126/science.373120. Science. 1979. PMID: 373120 Review.
-
Processing of tRNA in prokaryotes and eukaryotes.CRC Crit Rev Biochem. 1984;17(1):45-71. doi: 10.3109/10409238409110269. CRC Crit Rev Biochem. 1984. PMID: 6094100 Review.
Cited by
-
The ovalbumin gene: cloning and molecular organization of the entire natural gene.Proc Natl Acad Sci U S A. 1979 May;76(5):2253-7. doi: 10.1073/pnas.76.5.2253. Proc Natl Acad Sci U S A. 1979. PMID: 287066 Free PMC article.
-
tRNA biology charges to the front.Genes Dev. 2010 Sep 1;24(17):1832-60. doi: 10.1101/gad.1956510. Genes Dev. 2010. PMID: 20810645 Free PMC article. Review.
-
Yeast tRNA3Leu gene transcribed and spliced in a HeLa cell extract.Proc Natl Acad Sci U S A. 1981 Oct;78(10):5963-7. doi: 10.1073/pnas.78.10.5963. Proc Natl Acad Sci U S A. 1981. PMID: 6796956 Free PMC article.
-
Transfer RNA splicing in Saccharomyces cerevisiae: defining the substrates.Nucleic Acids Res. 1984 Dec 21;12(24):9367-82. doi: 10.1093/nar/12.24.9367. Nucleic Acids Res. 1984. PMID: 6096826 Free PMC article.
-
Structure of a yeast non-initiating methionine-tRNA gene.Nucleic Acids Res. 1980 May 10;8(9):1975-86. doi: 10.1093/nar/8.9.1975. Nucleic Acids Res. 1980. PMID: 6253952 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
