Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar 24:14:100293.
doi: 10.1016/j.fochx.2022.100293. eCollection 2022 Jun 30.

Dietary lysophosphatidylcholine regulates diacylglycerol, cardiolipin and free fatty acid contents in the fillet of turbot

Houguo Xu  1   2 , Xing Luo  1 , Yuliang Wei  1   2 , Mengqing Liang  1   2
Affiliations

Dietary lysophosphatidylcholine regulates diacylglycerol, cardiolipin and free fatty acid contents in the fillet of turbot

Houguo Xu et al. Food Chem X. .

Abstract

Lysophosphatidylcholine (LPC) has been widely used as emulsifier in animal feeds to enhance the lipid utilization. However, the effects of LPC on fillet quality has rarely been known. The present study was the first time to investigate the response of fish muscle lipidomics to dietary LPC supplementation. Turbot muscle samples were collected after a 56-day feeding trial where the experimental diet contained 0 or 0.25% LPC. Targeted tandem mass spectrometry was used in the lipidomic analysis. A total of 62 individual lipids (58 up-regulated and 7 down-regulated by LPC) showed significant difference in concentration in response to dietary LPC. Most of these differentially abundant lipids were diacylglycerol, free fatty acid and cardiolipin, and they all were up-regulated by dietary LPC. However, LPC exerted only marginal effects on muscle fatty acid composition and lipid content. The effects of dietary LPC on fillet lipid composition cannot be neglected in fish product evaluation.

Keywords: BMP, bis(monoacylglycero)phosphate; CE, cholesteryl ester; CL, cardiolipin; Cer, ceramide; Cho, cholesterol; DAG, diacylglycerol; Dietary regulation; FFA, free fatty acid; GM3, monosialodihexosyl ganglioside; GalCer, galactosylceramide; GluCer, glucosylceramide; LPA, lysophosphatidic acid; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; LPI, lysophosphatidylinositol; LPS, lysophosphatidylserine; LacCer, lactosylceramide; Lipidomics; Lysophospholipid; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; S1P, sphingosine 1-phosphate; SL, sulfolipid; SM, sphingomyelin; Scophthalmus maximus; Sph, sphingosine; TAG, triacylglycerol.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Heatmap of lipid class. The average concentration of a lipid metabolite was standardized to be 0. Higher concentration than the average was labeled as orange, and lower concentration was labeled as purple. The color value as indicated in the right color bar means fold of standard deviation distant to the average concentration. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Fig. 2
Fig. 2
Heatmap of identified diacylglycerols (DAG). The average concentration of a lipid metabolite was standardized to be 0. Higher concentration than the average was labeled as orange, and lower concentration was labeled as purple. The color value as indicated in the right color bar means fold of standard deviation distant to the average concentration. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Fig. 3
Fig. 3
Heatmap of identified free fatty acids (FFA). The average concentration of a lipid metabolite was standardized to be 0. Higher concentration than the average was labeled as orange, and lower concentration was labeled as purple. The color value as indicated in the right color bar means fold of standard deviation distant to the average concentration. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Fig. 4
Fig. 4
Heatmap of identified cardiolipin (CL). The average concentration of a lipid metabolite was standardized to be 0. Higher concentration than the average was labeled as orange, and lower concentration was labeled as purple. The color value as indicated in the right color bar means fold of standard deviation distant to the average concentration. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
See this image and copyright information in PMC

References

    1. Adhami B., Amirkolaei A.K., Oraji H., Kazemifard M., Mahjoub S. Effects of lysophospholipid on rainbow trout (Oncorhynchus mykiss) growth, biochemical indices, nutrient digestibility and liver histomorphometry when fed fat powder diet. Aquaculture Nutrition. 2021;27(6):1779–1788. doi: 10.1111/anu.13315. - DOI
    1. Association of Official Analytical Chemists (AOAC) 17th ed. Association of Official Analytical Chemists; Arlington, USA: 2000. Official Methods of Analysis of the Association of Official Analytical Chemists International.
    1. Bergman A.M., Trushenski J.T., Drawbridge M. Addition of emulsifiers to hydrogenated soybean oil-based feeds for yellowtail. North American Journal of Aquaculture. 2018;80(1):13–23. doi: 10.1002/naaq.10011. - DOI
    1. Fayyaz, S., Japtok, L., & Kleuser, B. (2014). Divergent role of sphingosine 1-phosphate on insulin resistance. Cellular Physiology and Biochemistry, 34(1), 134-147. doi: 10.1159/000362990. - PubMed
    1. Ferrara P.J., Verkerke A.R.P., Maschek J.A., Shahtout J.L., Siripoksup P., Eshima H.…Funai K. Low lysophosphatidylcholine induces skeletal muscle myopathy that is aggravated by high-fat diet feeding. FASEB Journal. 2021;35(10) doi: 10.1096/fj.202101104R. - DOI - PMC - PubMed

LinkOut - more resources