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. 2022:2450:245-261.
doi: 10.1007/978-1-0716-2172-1_13.

Studying Xenacoelomorpha WBR Using Isodiametra pulchra

Bernhard Egger  1
Affiliations

Studying Xenacoelomorpha WBR Using Isodiametra pulchra

Bernhard Egger. Methods Mol Biol. 2022.

Abstract

Xenacoelomorpha are a phylogenetically and biologically interesting, but severely understudied group of worm-like animals. Among them, the acoel Isodiametra pulchra has been shown to be amenable to experimental work, including the study of stem cells and regeneration. The animal is capable of regenerating the posterior part of the body, but not its head. Here, methods such as nucleic acid extractions, in situ hybridisation, RNA interference, antibody and cytochemical stainings, and the general handling of the animals are presented.

Keywords: Acoela; Anesthesia; Antibody stainings; In situ hybridization; Isodiametra; Neoblast stem cells; Phalloidin; RNA and DNA extraction; Regeneration.

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