Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2450:335-346.
doi: 10.1007/978-1-0716-2172-1_17.

In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis

Chiaki Kojima  1 , Noriko Funayama  2
Affiliations

In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis

Chiaki Kojima et al. Methods Mol Biol. 2022.

Abstract

Sponges (Porifera) are a large phylum that includes an enormous number of species. They are classified into four classes. Among these four classes, class Demospongiae is the largest and contains more than 90% of sponge species. In the last decade, methodologies for molecular studies and sequencing resources in sponge biology have dramatically advanced and made it possible to clearly define particular types of cells based on the genes they are expressing. Here we describe in detail the method of high-resolution WISH (whole mount in situ hybridization) and dual color fluorescent detection of in situ hybridization (dual color FISH) that we have established to detect particular types of cells, especially their stem cells known as archeocytes, in juveniles of freshwater demosponge, E. fluviatilis.

Keywords: Archeocyte; FISH; Musashi; Piwi; Porifera; Stem cells; WISH.

PubMed Disclaimer

References

    1. Funayama N, Nakatsukasa M, Mohri K, Masuda Y, Agata K (2010) Piwi expression in archeocytes and choanocytes in demosponges: insights into the stem cell system in demosponges. Evol Dev 12:275–287 - PubMed
    1. Funayama N (2010) The stem cell system in demosponges: insights into the origin of somatic stem cells. Dev Growth Differ 52:1–14 - PubMed
    1. Funayama N (2013) The stem cell system in demosponges: suggested involvement of two types of cells: Archeocytes (active stem cells) and choanocytes (food-entrapping flagellated cells). Dev Genes Evol 223:23–38 - PubMed
    1. Funayama N (2018) The cellular and molecular bases of the sponge stem cell systems underlying reproduction, homeostasis and regeneration. Int J Dev Biol 62:513–525 - PubMed
    1. Simpson TL (1984) The cell biology of sponges. Springer-Verlag, New York

Publication types