Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2450:373-398.
doi: 10.1007/978-1-0716-2172-1_20.

Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry

Wanda Buzgariu  1 , Jean-Pierre Aubry-Lachainaye  2 , Brigitte Galliot  3
Affiliations

Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry

Wanda Buzgariu et al. Methods Mol Biol. 2022.

Abstract

The freshwater Hydra polyp is a versatile model to study whole-body regeneration from a developmental as well as a cellular point of view. The outstanding regenerative capacities of Hydra are based on its three populations of adult stem cells located in the central body column of the animal. There, these three populations, gastrodermal epithelial, epidermal epithelial, and interstitial, continuously cycle in homeostatic conditions, and their activity is locally regulated after mid-gastric bisection. Moreover, they present an unusual cycling behavior with a short G1 phase and a pausing in G2. This particular cell cycle has been studied for a long time with classical microscopic methods. We describe here two flow cytometry methods that provide accurate and reproducible quantitative data to monitor cell cycle regulation in homeostatic and regenerative contexts. We also present a cell sorting procedure based on flow cytometry, whereby stem cells expressing a fluorescent reporter protein in transgenic lines can be enriched for use in applications such as transcriptomic, proteomic, or cell cycle analysis.

Keywords: Adult stem cells; Cell cycle; Cell cycle analysis; Click-iT EdU labeling of regenerating animals; Epithelial stem cells; Flow cytometry; GFP cell sorting; Hydra; Interstitial stem cells.

PubMed Disclaimer

References

    1. Steele RE (2002) Developmental signaling in Hydra: what does it take to build a “simple” animal? Dev Biol 248(2):199–219. pii:S001216060290744X - PubMed
    1. Bosch TC (2007) Why polyps regenerate and we don’t: towards a cellular and molecular framework for Hydra regeneration. Dev Biol 303(2):421–433. https://doi.org/10.1016/j.ydbio.2006.12.012 doi: 10.1016/j.ydbio.2006.12.012. - DOI - PubMed
    1. Galliot B, Ghila L (2010) Cell plasticity in homeostasis and regeneration. Mol Reprod Dev 77(10):837–855. https://doi.org/10.1002/mrd.21206 doi: 10.1002/mrd.21206. - DOI - PubMed
    1. Galliot B (2012) Hydra, a fruitful model system for 270 years. Int J Dev Biol 56(6–8):411–423. https://doi.org/10.1387/ijdb.120086bg doi: 10.1387/ijdb.120086bg. - DOI - PubMed
    1. Vogg MC, Galliot B, Tsiairis CD (2019) Model systems for regeneration: Hydra. Development 146(21):dev177212. https://doi.org/10.1242/dev.177212 doi: 10.1242/dev.177212. - DOI - PubMed

Publication types

LinkOut - more resources