Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022:2450:419-436.
doi: 10.1007/978-1-0716-2172-1_22.

Gene Manipulation in Hydractinia

Eleni Chrysostomou  1 , Febrimarsa  1 , Timothy DuBuc  2 , Uri Frank  3
Affiliations

Gene Manipulation in Hydractinia

Eleni Chrysostomou et al. Methods Mol Biol. 2022.

Abstract

The ability to regenerate lost body parts is irregularly distributed among animals, with substantial differences in regenerative potential between and within metazoan phyla. It is widely believed that regenerative animal clades inherited some aspects of their capacity to regenerate from their common ancestors but have also evolved new mechanisms that are not shared with other regenerative animals. Therefore, to gain a broad understanding of animal regenerative mechanisms and evolution, a broad sampling approach is necessary. Unfortunately, only few regenerative animals have been established as laboratory models with protocols for functional gene studies. Here, we describe the methods to establish transgenic individuals of the marine cnidarian Hydractinia. We also provide methods for transient gene expression manipulation without modifying the genome of the animals.

Keywords: CRISPR-Cas9; Hydractinia; Transgenesis.

PubMed Disclaimer

References

    1. Zapata F, Goetz FE, Smith SA, Howison M, Siebert S, Church SH, Sanders SM, Ames CL, McFadden CS, France SC, Daly M, Collins AG, Haddock SHD, Dunn CW, Cartwright P (2015) Phylogenomic analyses support traditional relationships within Cnidaria. PLoS One 10(10):e0139068. https://doi.org/10.1371/journal.pone.0139068 doi: 10.1371/journal.pone.0139068. - DOI - PMC - PubMed
    1. Technau U, Steele RE (2011) Evolutionary crossroads in developmental biology: Cnidaria. Development 138:1447–1458. https://doi.org/10.1242/dev.048959 doi: 10.1242/dev.048959. - DOI - PMC - PubMed
    1. Bosch TC, Klimovich A, Domazet-Loso T, Grunder S, Holstein TW, Jekely G, Miller DJ, Murillo-Rincon AP, Rentzsch F, Richards GS, Schroder K, Technau U, Yuste R (2017) Back to the basics: cnidarians start to fire. Trends Neurosci 40(2):92–105. https://doi.org/10.1016/j.tins.2016.11.005 doi: 10.1016/j.tins.2016.11.005. - DOI - PMC - PubMed
    1. Chang ES, Neuhof M, Rubinstein ND, Diamant A, Philippe H, Huchon D, Cartwright P (2015) Genomic insights into the evolutionary origin of Myxozoa within Cnidaria. Proc Natl Acad Sci U S A 112(48):14912–14917. https://doi.org/10.1073/pnas.1511468112 doi: 10.1073/pnas.1511468112. - DOI - PMC - PubMed
    1. Holstein TW, Hobmayer E, Technau U (2003) Cnidarians: an evolutionarily conserved model system for regeneration? Dev Dyn 226:257. https://doi.org/10.1002/dvdy.10227 doi: 10.1002/dvdy.10227. - DOI - PubMed

Publication types

LinkOut - more resources